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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | GL261 is a murine glioma cell line originally induced by the intracranial implantation of methylcholanthrene pellets into C57BL/6 mice. It currently stands as the most widely utilized syngeneic, immunocompetent model in the study of Glioblastoma Multiforme (GBM). GL261 cells exhibit a mixed morphology displaying characteristics of both fibroblasts and epithelial cells, effectively mimicking the aggressive growth, infiltrative properties, and immunosuppressive microenvironment characteristic of human GBM. Given its syngeneity with the C57BL/6 mouse strain, this model has become the preferred choice for investigating tumor-immune system interactions, evaluating the efficacy of immune checkpoint inhibitors (such as anti-PD-1/CTLA-4 antibodies), and testing novel dendritic cell vaccines or CAR-T cell therapies within the central nervous system. |
| Tissue | Brain |
| Disease | Glioma; Glioblastoma Multiforme (GBM) |
| Morphology | Spindle-shaped; Fibroblastic |
| Gender | Not specified (derived from C57BL/6 mouse) |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Glioblastoma pathogenesis and neuro-oncology research 2. Development of syngeneic orthotopic and subcutaneous brain tumor models 3. Evaluation of immunotherapies and vaccine strategies for CNS tumors 4. Study of the blood-brain barrier (BBB) and tumor-infiltrating lymphocytes (TILs) 5. Testing of radiotherapy and chemotherapy combination protocols |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in syngeneic C57BL/6 mice |
| Immunogenic Profile | Expresses MHC class I; relatively low levels of MHC class II |
| Genetic Profile | TP53 mutation; K-ras mutation; partially mimics human mesenchymal GBM subtype |
| Growth Kinetics | Rapid doubling time; consistent intracranial tumor progression |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically requiring 3 to 7 minutes). 4. Add complete growth medium to neutralize the trypsin, and gently pipette to create a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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