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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | The G422 mouse glioma cell line is a glioblastoma cell line derived from mouse brain tissue. It originated from an astrocytoma induced by the intracranial implantation of methylcholanthrene into Km mice; following 120 passages, this tumor ultimately transformed into a glioma. G422 cells exhibit highly malignant characteristics, specifically manifesting as rapid intracranial growth, extreme invasiveness into the surrounding brain parenchyma, and a resulting high mortality rate in host animals. This cell line serves as a critical tool for investigating the biological properties of malignant gliomas, assessing the ability of anti-tumor drugs to penetrate the blood-brain barrier (BBB), and evaluating the efficacy of surgical interventions, radiotherapy, and immunotherapy in immunocompetent hosts. |
| Tissue | Brain |
| Disease | Glioma; Glioblastoma |
| Morphology | Spindle-shaped; Polygonal |
| Gender | Not specified |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent (can also be maintained as an intracranial or subcutaneous tumor model) |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Malignant glioma pathogenesis and invasive growth research 2. Development of syngeneic orthotopic brain tumor models 3. Evaluation of drug delivery across the blood-brain barrier (BBB) 4. Screening for novel anti-glioma chemotherapeutics and traditional medicines 5. Study of tumor-associated immune suppression in the central nervous system |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in syngeneic mice (e.g., Kunming or BALB/c) |
| Growth Kinetics | Rapid doubling time; fast progression of intracranial mass effect |
| Phenotype | Maintains high expression of glial fibrillary acidic protein (GFAP) in vivo |
| Genetic Profile | Characteristic of high-grade malignant glioblastoma |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 1.0 to 2.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 2 to 5 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00761 | Luciferase Reporter Cell Line - G422 | Inquiry |
| CSC-RR00762 | GFP Reporter Cell Line - G422 | Inquiry |
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