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Gene families or biological pathways usually contain several related genes that collaborate in the same role. Increasing demand in loss-of-function screens has warranted the need for a multiplexed gene knock out cell line. Multiplexing with CRISPR/Cas9 can accelerate genome engineering efforts in biopharmaceutical research and drug discovery.
Creative Biogene provides additional rounds of transformation and selection until every targeted gene is knocked out. Mammalian cells exposed to two or three gRNAs in turn resulted in the generation of cell lines harboring double or triple gene knockouts, that is, the successful disruption of two or three genes. All multiplexed knockout events were obtained with DNA mutations in targeted genes, and harbored the expected knockout phenotype(s). Investigation of entire gene families or biological pathways is enabled by our dual/triple gene knock out cell line generation service.
The KO status of each cell line is thoroughly tested as follows:
Our services include:
Multiplexed Gene Knock Out Cell Line Generation Service Case
|Apoptosis||BAX/BAK||Human BAX/BAK Knockout Cell Line-DLD-1|
|Human BAX/BAK Knockout Cell Line-SW48|
|GS/BAX||GS/BAX Knockout Cell Line-CHO-K1|
|GS/Bax/Bak||GS/Bax/Bak1 Knockout Cell Line-CHO-K1|
|AKT1/AKT2||Human AKT1/AKT2 Knockout Cell Line-HCT116|
|Cell cycle||CDKN1A/BBC3||Human CDKN1A/BBC3 Knockout Cell Line-HCT116|
|CDK2/TP53||Human CDK2/TP53 Knockout Cell Line-HCT116|
|DNA repair||LIG4/XRCC6||Human XRCC6/LIG4 Knockout Cell Line-HCT116|
|PRKDC/XRCC6||Human PRKDC/XRCC6 Knockout Cell Line-HCT116|
|Rho/ROCK||ROCK1/ROCK2||ROCK1/ROCK2 Knockout Cell Line-NIH-3T3|