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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | OVCAR-3 cells are a human ovarian cancer cell line established from malignant ascites of a 60-year-old Caucasian female patient with progressive ovarian adenocarcinoma that was refractory to treatment with cyclophosphamide, doxorubicin, and cisplatin. OVCAR-3 cells are known to express high levels of estrogen receptor (ER) and progesterone receptor (PR) and are sensitive to hormones such as estradiol and progesterone. They also express high levels of cancer antigen 125 (CA-125), a marker for ovarian cancer. OVCAR-3 cells are epithelial in morphology and are characterized by high growth potential in vitro and the ability to form tumors in immunodeficient mice. OVCAR-3 cells are widely used in drug resistance studies, particularly those involving DNA damage response biomarkers, homologous recombination repair and overall cell cycle dynamics, cancer cell biology, and gene expression studies. |
| Tissue | Ovary |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 60 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Screening 3. Chemotherapy Resistance 4. Genetic and Molecular Studies 5. Biomarker Discovery 6. In Vitro Studies 7. Xenograft Models 8. Immunotherapy Research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The OVCAR3 cell line was used to create a xenograft mouse model that was used to monitor inhibition of tumor growth in vivo by alternative treatment strategies (e.g., vitamin supplementation with EB1089), gene silencing (e.g., claudin-3 siRNA), or small molecules targeting early relapse due to lack of HER2 amplification (e.g., trastuzumab). |
| Characteristics | |
| Karyotype | The cell line is an aneuploid human female with a chromosome number in the hypotriploid to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are noticeably absent. |
| Receptors Expressed | Androgen, estrogen, progesterone |
| Isoenzymes | G6PD, B, PGM1, 1, PGM3, 1, ES-D, 1, AK-1, 1, GLO-1, 1 |
| Tumorigenic | Yes, in nude mice |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old medium from adherent cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Let the cells incubate at room temperature for 8-10 minutes to detach them. 4. After incubation, gently mix the cells with 10 ml of medium to resuspend them, and then centrifuge at 300 x g for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 20%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | 55% basal medium + 40% FBS + 5% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO01213 | Cas9 Stable Cell Line - OVCAR-3 | Inquiry |
| CSC-RR00702 | Luciferase Reporter Cell Line - OVCAR-3 | Inquiry |
| CSC-RR01014 | GFP/Luc Reporter Cell Line - OVCAR-3 | Inquiry |
| CSC-RR0113 | RFP Stable Cell Line-OVCAR3 | Inquiry |
| CSC-RR0467 | GFP Stable Cell Line - OVCAR3 | Inquiry |
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