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Virus-like Particles (VLPs) represent a new generation of gene delivery vectors that are redefining technical standards in the gene therapy field by combining the high efficiency of viral vectors with the safety profile of non-viral vectors. Based on over a decade of deep expertise in molecular biology and nanobiotechnology, Creative Biogene has established a complete VLP gene delivery service system dedicated to providing one-stop solutions from concept design to preclinical validation for research institutions, biotechnology companies, and pharmaceutical enterprises worldwide.
Creative Biogene's service starts with an in-depth needs analysis and technical feasibility assessment, integrating target nucleic acid types, cell characteristics, and application scenarios to customize optimal VLP vector solutions. The design process considers scaffold selection, cargo loading, targeting strategies, and production optimization to balance functionality and cost.
Efficient nucleic acid loading is the core of VLP delivery systems. We have established three strategyies based on biological fusion, chemical coupling, physical adsorption, and self-assembly assistance to meet the loading requirements of different types of nucleic acids.
Nucleic Acid Loading Strategies
Protein Fusion
Fuses nucleic acid-binding domains (e.g., NC, RBP, DBP) with VLP structural proteins for efficient, directional packaging—ideal for large cargos like Cas9/gRNA.
Chemical Coupling
Uses mild bioorthogonal reactions (e.g., click chemistry, Schiff base) to covalently link nucleic acids (e.g., siRNA, miRNA) with VLPs. Supports various chemistries including amine-carboxyl, thiol-maleimide, and azide-alkyne.
Physical Adsorption
Applies electrostatic, hydrophobic, and hydrogen bonding for rapid, non-covalent loading. Optimized via pH, salt strength, and additives to enhance stability and efficiency.
We have developed a standardized service workflow to ensure high-quality, on-time project delivery.
1Needs Analysis: Our technical team engages with clients to understand the project background, goals, and budget, while assessing feasibility and risks to inform solution design.
2Solution Design: Based on the analysis, we design a detailed experimental plan covering VLP vector selection, construction strategy, production process, quality standards, and validation, with all technical details confirmed with the client.
3Experimental Implementation: We carry out VLP construction, production, purification, and validation according to the agreed plan, maintaining strict quality control at every step.
4Data Analysis: Experimental data is thoroughly analyzed to assess physicochemical properties, biological activity, and safety, providing objective technical conclusions.
5Report Delivery: A comprehensive report is delivered, including methods, data, analysis, and recommendations, along with SOPs and usage guidelines.
Through continuous technological innovation and market expansion, Creative Biogene is committed to becoming a globally leading VLP gene delivery technology service provider, contributing our professional expertise and technical wisdom to the development of human health. Please contact us if you have any needs.
Q: Is VLP delivery suitable for in vivo gene therapy?
A: Currently, our services focus on in vitro research and preclinical animal models. In vivo applications require further optimization of immunogenicity and scale-up production processes. We recommend that customers discuss feasibility in detail with the technical team at the project initiation stage.
Q: Can large DNA fragments exceeding 10 kb be delivered?
A: Current technology typically has a DNA loading capacity of less than 8 kb. For large fragment delivery needs (such as Cas12a systems), we can provide plasmid splitting or multi-VLP co-delivery solutions, but additional compatibility validation is required.
Q: How long does it take from needs submission to obtaining data?
A: The Standard project cycle is 6-8 weeks, including design, production, validation, and report generation. Complex projects (such as novel VLP construction) may extend to 12 weeks. The technical team will provide detailed timelines at project initiation.
Q: Do you support optimization of customer-owned VLP systems?
A: Yes. We provide process development services, including purification process optimization, loading efficiency improvement, and stability testing (such as freeze-thaw cycle tolerance). Basic constructs and target parameters need to be provided.
Q: How do you ensure consistency between different batches of VLPs?
A: We employ standardized production processes and strict quality control (such as SEC purity >90%, DLS uniformity detection), providing batch-to-batch comparison data (such as particle size distribution and loading efficiency). Customers can request reserved backup samples for repeat experiments.
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