MEF Cell Line

Brief Introdution
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	Mouse embryonic fibroblasts (MEFs) are fibroblasts prepared from mouse embryos. This cell can be used as a feeder layer to support the growth of embryonic stem (ES) cells and maintain ES cells in an undifferentiated state. MEFs exhibit a spindle shape when cultured in vitro, a characteristic characteristic of fibroblasts. MEFs are a limited cell line. After several spreads, MEFs will age and eventually die. Still, researchers can use a variety of strategies, such as viral infection or repeated transmission, to immortalize MEF cells, which allows MEFs to grow indefinitely, albeit with some changes in properties. MEF is widely used in life science research, especially stem cell biology.
Tissue	Embryo
Morphology	Fibroblast
Strain	C57BL/6
Gender	Male and female mixed
Age	14 days gestation
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Gene functions studies 2. Cancer research 3. Stem cell research 4. Drug testing 5. Protein Interaction studies
Shipped in	Dry ice
Storage Temperature	−196°C
Additional Info	In 1962, two researchers at New York University, George Todaro and Howard Green, immortalized MEF through repeated propagation. These cells developed into the commonly used cell line NIH 3T3. MEFs treated with mitomycin or gamma rays (this treatment stops MEFs from mitosis) are widely used as feeder layers in embryonic stem cell cultures because they can mimic the microenvironment in the embryo. In 2006, Shinya Yamanaka reprogrammed MEFs into iPSCs by introducing 4 factors, which was remarkable in the development of stem cell biology.
Culture Conditions and Handling
Subculturing	To ensure the highest level of viability, be sure to heat the culture medium and trypsin/EDTA to 37°C before using it with cells. Cells should be divided when they reach confluence. Splitting is recommended based on a seed density of 2 X 104 cells/cm². 1. Remove and discard the culture medium. 2. Remove all traces of serum by briefly washing the cell layer with 1XPBS solution, which contains trypsin inhibitors. 3. Add 0.25% trypsin-0.53 mM EDTA solution to the flask and incubate for 2 minutes. Tap the flask gently and observe the cells under an inverted microscope. Cells typically detach within 2 to 3 minutes. 4. Add an equal volume of growth medium and rinse the flask surface to detach all cells. Gently pipetting up and down will break up the cell clumps. 5. Transfer all cells to a centrifuge bottle or tube and centrifuge at 270 x g for 5 minutes. 6. Remove and discard supernatant 7. Add 10 mL of complete growth medium to the cell pellet and gently resuspend the cells with a 10 mL pipette. 8. Add more complete growth medium to the cell suspension as needed to plate cells at approximately 0.8 X 104 cells/cm². 9. Place the flask in an incubator at 37°C with 5% CO₂ in air.
Subcultivation Ratio	The ratio of 1:5 to 1:8 is recommended.
Culture Medium	Dulbecco's Modified Eagle's Medium + 15% Foetal Bovine Serum (FBS)
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%;Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 40% (v/v) FBS and 10% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
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* For research use only. Not intended for any clinical use.

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