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Plant Expression Vector Construction

Gene cloning and vector construction are fundamental techniques in plant genetic engineering. Efficient and well-designed vectors are essential for successful plant transformation, enabling precise control of gene expression and stable integration into the plant genome. The selection of appropriate expression constructs is crucial not only for improving agronomic traits but also for exploring gene function, dissecting regulatory mechanisms, and performing promoter activity studies.

The processes of plant genetic engineeringFigure 1. The processes of plant genetic engineering.

Creative Biogene specializes in plant genetic engineering and molecular biology, offering professional, customizable plant expression vector construction services. Our solutions support both academic research and applied agricultural biotechnology, providing high-quality vectors for functional genomics, molecular breeding, and crop improvement projects.

Service Categories

01 Overexpression Vector Construction

We offer a variety of promoters to match different experimental goals:

  • Constitutive promoters: p35S, pUbi, pNos, ensuring continuous expression in most tissues.
  • Inducible promoters: pCab, pEr, allowing controlled expression under specific conditions.
  • Tissue-specific promoters: pRbcs, pGlu, pGt1, driving expression in targeted tissues such as leaves, seeds, or roots.

02 Gene Silencing Vector Construction

Our gene silencing vectors include:

  • RNAi vectors for stable, long-term silencing of target genes.
  • VIGS (virus-induced gene silencing) vectors for rapid, transient knockdown of gene expression.

03 Gene Knockout Vector Construction

CRISPR/Cas9-based knockout vectors with codon-optimized Cas9 sequences are available for precise gene editing in plants.

  • Dicot plants: sgRNA expression driven by AtU6 promoter.
  • Monocot plants: sgRNA expression driven by OsU3 or OsU6 promoters.

04 Promoter Activity Analysis Vectors

Vectors are designed to fuse target promoters with reporter genes, enabling:

  • Histochemical staining.
  • Quantitative and qualitative analysis of promoter activity under different tissues or environmental conditions.

05 Complementary Expression Vectors

Vectors with native promoters drive gene expression for functional complementation studies, supporting:

  • Rescue of mutant phenotypes.
  • Functional validation of genes of interest.

Applications

  • Overexpression vectors: Boost target gene expression for protein production, metabolic pathway engineering, or functional characterization.
  • RNAi vectors: Silence genes to analyze functional roles and observe phenotypic changes.
  • Promoter deletion vectors: Study promoter architecture and regulatory elements using reporter gene assays.
  • CRISPR/Cas9 vectors: Create knockout lines to investigate gene function and improve agronomic traits.
  • Complementary expression vectors: Validate gene function through phenotypic rescue experiments.

Workflow of Our Plant Expression Vector Construction Service

Service Process

1Customer order placement: Submit gene sequence or GenBank accession number.

2Project confirmation: Technical team reviews sequence and experimental requirements.

3Gene cloning/synthesis: ORFs or customized sequences are cloned or synthesized.

4Expression vector construction: Overexpression, silencing, knockout, or reporter vectors are assembled.

5Plant transformation: Agrobacterium-mediated transformation can be performed if required.

6Plant identification: Screening and verification of transgenic plants.

7Sample delivery: Plasmids, strains, and detailed documentation are provided.

Service Advantages

  • End-to-end assay coverage: From cell culture to ELISA, qPCR, and PERT, we provide a full suite of tests to ensure no replication-competent lentivirus escapes detection.
  • Unmatched sensitivity and reliability: Multi-method validation delivers ultra-sensitive results you can trust for both research and regulatory submission.
  • Accelerated timelines with confidence: Streamlined workflows shorten turnaround while maintaining data accuracy and regulatory readiness.
  • Global regulatory alignment: Our testing meets the highest standards, fully compliant with FDA and EMA guidance for gene therapy safety.
  • Actionable QC deliverables: Receive not just raw data, but complete reports with expert analysis and recommendations to guide your next step.

Service Notes

Submit Your Sequence

Provide the ORF sequence or GenBank accession number to kickstart your customized vector project.

Fast & Reliable Timeline

Our standard delivery takes 15–21 business days, depending on vector type and project complexity, ensuring timely progress for your research.

Complete Project Deliverables

Receive one plasmid or strain sample, along with a detailed completion report including vector map, sequencing results, and practical usage instructions for seamless downstream applications.

Contact Us

With extensive experience and a comprehensive vector library, Creative Biogene provides flexible, efficient, and cost-effective plant expression vector solutions. Our services support gene functional studies, promoter activity analysis, molecular breeding, and biotechnological innovation, helping researchers achieve reliable results with confidence.

Related Service

Agrobacterium-mediated Transformation Service

Plant-based Protein Production Service

Plant RNA Fluorescent In Situ Hybridization (FISH) Service

Functional Analysis Service

* For research use only. Not intended for any clinical use.
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