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Plant Expression Vector Construction

Creative Biogene is an expert in plant genetic engineering and molecular biology. We have devoted many years of efforts in optimizing plant expression vectors to adapt to different plant species. We provide universal and customized plant expression vector construction services, covering from sequence optimization, gene synthesis/cloning, DNA fragment assembling, sequencing to plasmid preparation. With advanced technologies and highly experienced staffs, we will work closely with you to bring satisfied plant expression vector construction services.

Gene cloning and vector construction are routine procedures in plant genetic engineering. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. The generation of genetically modified crops with valued traits requires different combinations of expression elements to achieve optimum effects. In addition, a right choice of expression construct also plays an important role in research of gene function and promoter activity analysis.

The processes of plant genetic engineering Fig 1. The processes of plant genetic engineering

Service

  • Overexpression vector construction: Fully customizable vector that you can choose specific promoter like inducible promoter (pCab, pEr), constitutive promoter (p35s, pUbi, pNos) and tissue-specific promoter (pRbcs, pGlu, pGt1).
  • Silencing vector construction: RNAi vector for stable gene silencing. VIGS (virus-induced gene silencing) vector for transient gene silencing.
  • Gene knockout vector construction: The cas9 gene sequence has been codon optimized to improve the expression in plant. AtU6 promoter drives sgRNA expression in dicots and OsU3/OsU6 drives sgRNA in monocots.
  • Promoter activity analysis vector construction: Your target promoter will be fused to GusPlus gene for histochemical GUS staining use.
  • Complementary expression vector construction: Native promoter drives gene expression.

Highlights

  • Optimized: Our knowledge of expression vector and its elements help you optimizing the vector at the design stage.
  • Customized: With our capability in molecular biology we are able to make any building block for the vector you want.
  • Guaranteed: All sequences will be confirmed.

Creative Biogene's advanced molecular biology platform has provided hundreds of plant expression vectors to customers worldwide including pCAMBIA series, pEarleyGate series, pBI series, pRI series, pGreen series. These have been successfully used in gene overexpression, targeted expression, inducible expression, gene silencing, gene editing, gene stacking and promoter activity analysis projects. Importantly, all of our plasmid vectors are optimized for high copy number replication in E. coli and high-efficiency plant delivery and gene expression. Creative Biogene's goal is to provide you with the most affordable, high-quality custom vector construction and modification services to ensure your satisfaction in a timely and professional manner.

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