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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-Hep-1 is a permanent cell line derived from the ascites of a 52-year-old Caucasian male patient with hepatic adenocarcinoma. SK-Hep-1 has been widely used as a cell model for hepatocellular carcinoma (HCC) and hepatocyte biology. However, no liver-specific genes, including albumin and alpha and gamma fibrinogen, are expressed in SK-Hep-1 cells. In addition, SK-Hep-1 cells are significantly different from normal hepatocytes and other HCC cells by proteomic analysis. Impressively, SK-Hep1 cells are positive for the expression of endothelial markers, including von Willebrand factor (vWF) and endothelial leukocyte adhesion molecule-1 (ELAM-1). In addition, the ultrastructure of pinocytic vesicles and organelles consistent with Weibel-Palade bodies under transmission electron microscopy (TEM) suggest an endothelial origin of SK-Hep-1 cells. The cells can be used in cardiovascular disease research, toxicology, and immuno-oncology. |
| Tissue | Liver |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 52 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Screening and Development 3. Gene Expression Studies 4. Signal Transduction Pathways 5. Virus Research 6. Toxicology Testing |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The SK-HEP-1 cell line is used to create the CDX (cell line derived xenograft) SK-HEP-1 xenograft mouse model. The SK-HEP-1 xenograft model is used for monotherapy studies or anti-tumor activity studies of sphingosine kinase 2 inhibitors (e.g., ABC-294640) in combination with sorafenib (mechanism of action is through inhibition of the MAPK pathway; reduction of phosphorylated ERK levels). |
| Characteristics | |
| Karyotype | The cell line is aneuploid human (XX) with a chromosome number in the subtriploid range. Normal chromosomes N1, N13, N14, N15, and N16 are significantly underrepresented relative to other normal chromosomes, while chromosomes N7, N12, and N17 are often overrepresented in certain metaphases. |
| Isoenzymes | Me-2, 1-2, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Let the cells incubate at room temperature for 8-10 minutes to detach them. 4. After incubation, gently mix the cells with 10 ml of culture medium to resuspend them, and then centrifuge at 300 x g for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh culture medium, and transfer them to a new flask that already contains fresh culture medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
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