Caco-2 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	Caco-2 is an immortalized cell line of human colorectal adenocarcinoma cells. It was developed in 1977 by Jorgen Fogh at Sloan Kettering Cancer Institute. Primary colon tumors were isolated from a 72-year-old Caucasian male using explant culture techniques. Moderately differentiated adenocarcinoma consistent with colonic primary grade II develops in nude mice. It is mainly used as a model of the intestinal epithelial barrier. The Caco-2 cell line is heterogeneous and contains cells with slightly different properties. Therefore, it can be expected that culture conditions can select for the growth of subpopulations of cells, thereby producing a cellular model system with properties that may differ from the original cell line. Therefore, results obtained under similar experimental conditions in different laboratories may not be directly comparable.
Tissue	Colon
Disease	Colorectal Adenocarcinoma
Morphology	Epithelial-like
Gender	Male
Age	72 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	Drug permeability studies Drug metabolism studies Drug transport studies Drug-drug interaction studies 3D cell culture High-throughput screening
Shipped in	Dry ice
Storage Temperature	−196°C
Additional Info	Although derived from colon (large intestine) cancer, when cultured under specific conditions, the cells differentiate and polarize to a phenotype, morphology and function similar to the intestinal epithelial cells lining the small intestine. Polarized caco-2 cells express tight junctions, microvilli, and many enzymes and transporters unique to these enterocytes: peptidases, esterases, P-glycoproteins, uptake transporters for amino acids, bile acids, carboxylic acids, etc.
Characteristics
Karyotype	The stemline modal chromosome number is 96, the incidence rate is 16%, and the polyploidy rate is 3.2%. 10 common markers were detected. Normal N9 is absent, and N21 is lost in some cells. One to four small acrocentric chromosomes were detected. The Y chromosome with the bright distal q band was not detected by Q observation.
Tumorigenic	Yes, forms tumors in nude mice.
Genes Expressed	Keratin; retinoic acid binding protein 1; retinol binding protein 2
Expression Markers	This cell line expressed heat-stable enterotoxin and epidermal growth factor (EGF).
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. The recommended inoculum size is 1 X 10⁴ viable cells/cm². When cells are approximately 80% confluent, subculture at a cell concentration between 8 x 10⁴ and 1 x 10⁵ cells/cm². 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm² flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium.
Medium Renewal	1 to 2 times per week
Subcultivation Ratio	The ratio of 1:4 to 1:6 is recommended.
Culture Medium	EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%;Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO01170	Cas9 Stable Cell Line - Caco-2	Inquiry
CSC-RR00669	Luciferase Reporter Cell Line - Caco-2	Inquiry
CSC-RR00866	RFP/Luc Reporter Cell Line - CACO-2	Inquiry
CSC-RR00919	GFP Reporter Cell Line - CACO2	Inquiry
CSC-RT0096	TRPV1 Knockout Cell Line-caco-2	Inquiry
CSC-RT2586	Human SLC11A2 Knockout Cell Line-caco2	Inquiry

* For research use only. Not intended for any clinical use.

Quick Inquiry