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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | REH, established in 1974 as the first B cell precursor acute lymphoblastic leukemia (ALL) cell line, was derived from the peripheral blood of a 15 year-old female patient at relapse. REH has a near-diploid karyotype characterized by complex structural rearrangements, including several interchromosomal translocations. Among the features described in this cell line is a complex four-way translocation t(4;12;21;16) that results in the canonical subtype-defining ETV6-RUNX1 fusion gene and glucocorticoid (GC) resistance attributed to lack of a GC receptor. These REH cells are often used in research on immune system disorders and cancer. |
| Tissue | Peripheral blood |
| Disease | Acute lymphoblastic leukemia (ALL) |
| Morphology | Lymphoblast |
| Age | 15 years |
| Gender | Female |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Development Research 3. Genetic Studies 4. Immunotherapy Research 5. Biomarker Discovery 6. Toxicity Testing |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The REH cell line comes from a relapsed patient, making it particularly notable that several deletions identified in the study, affecting genes TBL1XR1 (146k deletion on 3q26.32), NR3C1 (205 kbp deletion on 5q31.3) and BTG1 (260kbp deletion on 12q21.33), have been shown to contribute to its GC drug resistance. |
| Characteristics | |
| Antigen Expression | CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Maintain the culture by regularly adding or replacing the medium. Start the culture at 2 x 10^5 cells/mL and maintain the cell concentration in the range of 1 x 10^5 to 1 x 10^6 cells/mL for optimal growth. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Fetal bovine serum supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0574 | GFP Reporter Cell Line - REH | Inquiry |
| CSC-RR0575 | Luciferase Reporter Cell Line - REH | Inquiry |
| CSC-RR0576 | GFP/Luc Reporter Cell Line - REH | Inquiry |
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