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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | UM-UC-3 is a human bladder cancer cell line derived from high-grade (grade 3) transitional cell carcinoma (urothelial carcinoma) of the bladder. Due to its highly invasive and aggressive growth characteristics, UM-UC-3 is one of the most commonly used models for bladder cancer research. A significant characteristic of UM-UC-3 cells is the absence of the PTEN and p53 tumor suppressor genes, and their proliferation depends on the EGFR signaling pathway. This cell line is an important tool for studying the molecular drivers of bladder cancer progression, epithelial-mesenchymal transition (EMT), and the screening of targeted therapies and chemotherapeutic agents. |
| Tissue | Urinary Bladder |
| Disease | Bladder carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | Not specified |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Bladder cancer invasion and metastasis studies 2. PTEN and p53 signaling pathway studies 3. Screening of therapeutic drugs for urothelial carcinoma 4. EGFR-targeted therapy resistance studies 5. Construction of orthotopic and subcutaneous xenograft models |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Karyotype | Aneuploid; highly rearranged chromosomal structure |
| Genetic Profile | PTEN null; TP53 mutation; RB1 wild-type |
| Receptors Expressed | High expression of Epidermal Growth Factor Receptor (EGFR) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently pipette until the cell suspension is a single-cell suspension. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | Eagle's Minimum Essential Medium (EMEM) or DMEM supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00857 | Luciferase Reporter Cell Line - UM-UC-3 | Inquiry |
| CSC-RR01018 | GFP/Luc Reporter Cell Line - UM-UC-3 | Inquiry |
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