Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
The team at Creative Biogene boasts extensive expertise in cell technology spanning several decades. We specialize in developing and providing top-tier cell-related products and services to support the research endeavors of clients worldwide.
Creative Biogene offers a diverse array of lysates derived from various cell lines, including HEK293, HEK293T, HeLa, insect cells, and more. These lysates are generated by introducing exogenous protein-encoding sequences into the cells through transfection or transduction, followed by homogenization using RIPA Lysis Buffer. Our collection of cell lysates serves as effective positive controls for validating newly generated antibodies targeting antigens from human cells, viruses, and other sources.
Advantages of Our Over-expression Cell Lysate
Over-expression Cell Lysate is a valuable research tool generated from cells overexpressing specific proteins of interest. It serves as a critical resource for various experimental applications. By utilizing our Over-expression Cell Lysates corresponding to specific proteins, researchers can advance their understanding of cellular processes, disease mechanisms, and therapeutic targets:
Case Study 1
Researchers aimed to investigate the potential neuroprotective role of human cytomegalovirus immediate-early protein pUL37 × 1 in Parkinson's disease (PD), focusing on its effects on mitochondrial dysfunction and apoptosis. They found that pUL37 × 1 induces Bax mitochondrial translocation and inactivation, leading to protection against apoptosis and cell death induced by staurosporine and 6-hydroxydopamine. Interestingly, this protection was not enhanced by silencing of Bax, suggesting a Bax-independent mechanism. Furthermore, pUL37×1 was found to increase glycolysis and induce mitochondrial hyperpolarization through a Bax-independent pathway involving activation of phosphofructokinase by a calcium-dependent pathway. Overall, these findings suggest that pUL37×1 may offer a novel neuroprotective strategy in diseases characterized by mitochondrial dysfunction and apoptotic pathways.
Figure 1. Researchers aimed to evaluate the protective effects of pUL37×1 over-expression against toxin-induced apoptosis and cell death in SH-SY5Y cells, with a focus on assessing changes in cytosolic cytochrome c levels and caspase-3 activity using cell lysate analysis. (Hong CT, et al., 2016)
Case Study 2
(α-isopropylmalate synthase) MtαIPMS, an enzyme in Mycobacterium tuberculosis, is a potential drug target for tuberculosis treatment. Cations have differential effects on its activation and inhibition, but the underlying mechanism remains unclear. Researchers conducted a comprehensive study on the effects of cations on the structure and function of MtαIPMS, a potential drug target for tuberculosis treatment. They discovered that various cations interacted with specific sites on the enzyme, leading to differential modulation of its structure and function. Inhibitors such as Zn2+ and Cd2+ directly interacted with the catalytic domain, causing unfolding or denaturation. On the other hand, the activator K+ interacted with the catalytic domain without significant structural effects. Additionally, divalent cations induced interaction between the regulatory and catalytic domains, resulting in structural cooperativity and potential modulation of enzyme activity.
Figure 2. MtαIPMS was over-expressed and purified, followed by SDS-PAGE analysis of the cell lysate containing the over-expressed MtαIPMS and the purified protein. Lanes 1–4 on the gel correspond to molecular weight markers, the supernatant of un-induced cell lysate, the supernatant of induced cell lysate, and the purified protein, respectively. (Singh K, et al., 2007)
Q: What advantages of Creative Biogene's Over-expression Cell Lysates?
A: Our lysates offer high-quality, ready-to-use solutions, ensuring high expression levels, batch consistency, and compatibility with various techniques, streamlining research.
Q: Contribution to research efficiency and cost-effectiveness?
A: By saving time and resources associated with cell line establishment, our lysates enhance research efficiency and reduce costs.
Q: Quality control measures for reliability?
A: Rigorous testing ensures the purity, integrity, and functionality of each batch, guaranteeing reliable and reproducible results.
Q: Are customization options available?
A: Yes, researchers can customize lysates based on format and target proteins, meeting specific research needs effectively.
Q: Comparison to other products?
A: Our lysates stand out for superior quality, reliability, and versatility, supported by continuous innovation and advancement.
| Cat.No. | Product Name | Price |
|---|---|---|
| CLOE-0001 | Human ADGRE5(His) HEK293 Cell Lysate | Inquiry |
| CLOE-0002 | Human ADGRE5(Fc) HEK293 Cell Lysate | Inquiry |
| CLOE-0003 | Human PRKAG1/B2/A1 Insect Cell Lysate | Inquiry |
| CLOE-0004 | Human PRKAG1/B1/A2 Insect Cell Lysate | Inquiry |
| CLOE-0005 | Human PRKAG1/B2/A2 Insect Cell Lysate | Inquiry |
| CLOE-0006 | Human PRKAG1/B1/A1 Insect Cell Lysate | Inquiry |
| CLOE-0007 | Human IL17A/F HEK293 Cell Lysate | Inquiry |
| CLOE-0008 | Human IL12B/23A HEK293 Cell Lysate | Inquiry |
| CLOE-0009 | Human B2M/CD1D HEK293 Cell Lysate | Inquiry |
| CLOE-0010 | Human B2M/CD1B HEK293 Cell Lysate | Inquiry |
| CLOE-0011 | Human CD3E/G HEK293 Cell Lysate | Inquiry |
| CLOE-0012 | Human IL12A/EBI3 HEK293 Cell Lysate | Inquiry |
| CLOE-0013 | Human CD3D/E HEK293 Cell Lysate | Inquiry |
| CLOE-0014 | Human ITGAV/B6 HEK293 Cell Lysate | Inquiry |
| CLOE-0015 | Human CD3D/E(Fc) HEK293 Cell Lysate | Inquiry |
Copyright © Creative Biogene. All rights reserved.