A-431 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	A-431 is a cell line that exhibits epithelial morphology and was isolated from the epidermis of an 85-year-old female patient with epidermoid carcinoma. When EGF binds to its receptor (EGFR) on the surface of A-431 cells, rapid tyrosine phosphorylation of the membrane protein occurs, initiating a series of intracellular signaling pathways. These pathways include the MAPK/ERK and PI3K/AKT pathways, which play key roles in regulating cell cycle progression, survival, and proliferation. EGFR stimulates cell proliferation at low concentrations, while at high concentrations it inhibits the growth of A-431 cells and induces their terminal differentiation. This dynamic response to EGFR emphasizes the utility of this cell line for exploring cell signaling pathways and the cell cycle in the context of cancer. In addition, A-431 cell-derived xenograft models are used to study tumor behavior in a living setting and to evaluate anticancer therapies.
Tissue	Skin; Epidermis
Disease	Epidermoid carcinoma
Morphology	Epithelial
Gender	Female
Age	85 days
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Drug testing and development 3. Signal transduction studies 4. Skin physiology and disease studies 5. Tumor Xenograft models
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	Treatment of A-431 cells with bradykinin reduced basal and EGF-induced EGFR phosphorylation. Treatment with Sertoli cell secretory growth factor (SCSGF) strongly induced cell proliferation. Stimulation of A-431 cells with phorbol esters induced the expression of the interleukin 1-related protein IL1H.
Characteristics
Karyotype	This is a hypertriploid human cell line. 36% of the cells have 74 chromosomes. The proportion of hyperploid cells is 1.0%.
Receptors Expressed	EGF-binding sites
Protein Expression	p53 positive
Isoenzymes	G6PD, B, PGM1, 1, PGM3, 1, ES-D, 1, Me-2, 0, AK-1, 1, GLO-1, 2
Tumorigenic	Yes, in immunosuppressed mice
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The volumes given are for 75 cm² flasks. For culture vessels of other sizes, increase or decrease the amount of dissociation medium required proportionally. 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 ml of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 ml of complete growth medium and gently aspirate the cells. 5. Add an appropriate amount of the cell suspension to a new culture vessel. 6. Incubate the culture at 37℃.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	The ratio of 1:3 to 1:8 is recommended.
Culture Medium	90% RPMI 1640 + 10% h.i. FBS
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO01161	Cas9 Stable Cell Line - A-431	Inquiry
CSC-RR00658	Luciferase Reporter Cell Line - A-431	Inquiry
CSC-RR01060	mScarlet/LUC Reporter Cell Line - A-431	Inquiry

* For research use only. Not intended for any clinical use.

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