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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SW620 is a human colon cancer cell line derived from metastatic sites of primary colon adenocarcinoma. The SW-620 tumorigenic epithelial cell line, obtained by A. Leibovitz from the lymph nodes of a 51-year-old white male patient with colorectal adenocarcinoma, is a suitable transfection host. The SW-620 cell line expresses carcinoembryonic antigen (CEA), transforming growth factor α, and stromelysin. Furthermore, these cells were negative for CSap and colon antigen 3 and positive for keratin by immunoperoxidase staining. SW-620 cells are critical for studying resistance mechanisms to apoptosis and anoikis, a form of programmed cell death critical for preventing metastasis. Studies using SW-620 colon cancer cells delved into proteomic analysis to understand changes in the proteome under different conditions, such as hypoxia. Hypoxic SW620 cells exhibit specific proteomic adaptations that contribute to chemoresistance. SW-620 cells exhibit high tumorigenic and metastatic abilities to form solid tumors in vivo. The SW620 xenograft model and the study of specific pathways such as the catenin pathway and the role of transcription factors such as cdx2 in colon adenocarcinoma cells have enriched the understanding of the molecular basis of colorectal cancer. |
| Tissue | Large intestine; Colon |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 51 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research and drug screening 2. Metastasis research 3. Genomic and proteomic studies 4. Biomarker identification 5. In vitro and in vivo studies 6. Signal transduction pathways research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | These SW620 cells synthesize small amounts of CEA and are highly tumorigenic in nude mice. The established cell line consists of small spherical and bipolar cells that resemble microvilli. Both SW-480 and SW-620 were established based on primary and secondary tumors in individual patients. SW-620 cells have a fibroblast-like appearance and xenografts form solid sheets and are more tumorigenic, metastatic and resistant to TNF-alpha and anti-Fas induced apoptosis as compared to its earlier stage SW480 counterpart, which is consistent with tumor progression. |
| Characteristics | |
| Oncogene | abl-; myc+; myb+; ras+; fos+; p53+; ros-; src-; sis+ |
| Antigen Expression | Blood type A; Rh+ |
| Karyotype | modal number = 50; range = 45 to 53 The number of chromosomes in the stem line is hyperdiploid, of which 2S component accounts for 12%, and 11 marker chromosomes were common to S metaphases. |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with Ca++/Mg++-free Dulbecco's Phosphate Buffered Saline (D-PBS) or 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:10 is recommended. |
| Culture Medium | L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 100%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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