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Cell line immortalization is a crucial tool in research, enabling indefinite cell proliferation. Immortalized cell lines, like HEK293 and NIH3T3, offer time and energy savings compared to frequent primary cell isolation. Immortalization can occur naturally or be induced by factors such as SV40 T antigen, human TERT, and HPV E6-E7. Replication-deficient lentiviruses, efficient gene delivery tools, are commonly used for immortalization, especially in challenging primary cells. Creative Biogene provides lentiviral particles containing genes like SV40 T, human TERT, and HPV-16 E6/E7 for establishing immortalized cell lines.
Figure 1. Schematic diagram of cell immortalization
Benefits of Our Cell Immortalization Lentiviral Particles
Cell Immortalization Lentiviral Particles Product List
Immortalizing lentiviruses efficiently introduce key factors into primary cells to enable unlimited proliferation without loss of function, avoiding repetitive sourcing/culture. Sustained factor expression lentivirally transforms cells bypassing senescence. The principle involves transduction to integrate immortalizing genes allowing selection/expansion of stable immortal subpopulations ideal for biomanufacturing and research. Our diverse range of lentiviral particles, especially the polyvalent immortalizing lentiviruses, serves as a versatile technology with broad applications:
Case Study 1
Acute lung injury (ALI) stands as a prevalent and critical clinical condition, with stem cell transplantation acknowledged as an effective strategy for mending damaged lung tissues. Researchers utilized lentiviral vectors to introduce overexpression of Hox A9 in mesenchymal stem cells (MSCs) to investigate their impact on lung repair in ALI. The distribution and expression of HoxA9 mRNA in cells were examined post-lentiviral transduction. The results indicated efficient and stable expression of HoxA9 in the cell nucleus. Additionally, the study assessed the proliferation and adhesion capabilities of MSC-Hox A9, revealing increased proliferation and significantly reduced inflammation-induced adhesion compared to normal MSCs.
Figure 1. Both the empty plasmid group and the HoxA9 group exhibited visible fluorescence, with the former distributed throughout the cells and the latter specifically localized in the nucleus. (Xin X, et al., 2017).
Case Study 2
Hepatocellular carcinoma (HCC) exhibits a significant mortality rate, and identifying an effective therapeutic target remains challenging. Researchers employed lentiviral vectors to investigate the role of nuclear receptor coactivator-3 (NCOA3) in hepatocellular carcinoma (HCC). NCOA3 was identified as a modulator of human telomerase reverse transcriptase (TERT) expression in HCC, binding to the TERT promoter and activating its transcription. NCOA3 promoted HCC cell growth and tumor progression by upregulating the TERT signaling pathway. Knockdown of NCOA3 inhibited HCC cell viability, and its interaction with SP1 on the TERT promoter was crucial. The study suggests that targeting the NCOA3-SP1-TERT signaling axis could be a promising therapeutic approach for HCC.
![Figure 2. HCC cells with stable NCOA3 overexpression were established by transfecting lentivirus pLV[Exp]-Puro-CMV-NCOA3-IRES: EGFP. NCOA3 knockdown led to reduced TERT mRNA and protein levels in HepG2 and Hep3B cells.](/upload/image/cell-immortalization-lentiviral-particles-2.jpg)
Figure 2. HCC cells with stable NCOA3 overexpression were established by transfecting lentivirus pLV[Exp]-Puro-CMV-NCOA3-IRES: EGFP. NCOA3 knockdown led to reduced TERT mRNA and protein levels in HepG2 and Hep3B cells. (Li W, et al., 2020).
Q: How do Cell Immortalization Lentiviral Particles work at a molecular level?
A: The lentiviral particles carry genetic elements, such as viral oncogenes or telomerase, which, when introduced into cells, override normal cellular senescence mechanisms, allowing cells to proliferate beyond their usual limits.
Q: What are the key genetic elements or factors included in these lentiviral particles for cell immortalization?
A: Commonly included elements are viral oncogenes like SV40 large T antigen or human telomerase reverse transcriptase (hTERT), which contribute to cell immortalization.
Q: In what experimental scenarios is the use of Cell Immortalization Lentiviral Particles particularly beneficial?
A: These particles are valuable in scenarios where extended cell culture passages are required, such as long-term studies, high-throughput screening, or experiments that demand continuous cell proliferation.
Q: How can researchers assess the success of cell immortalization induced by lentiviral particles?
A: Researchers can monitor the extension of cell lifespan, maintenance of proliferative capacity, and confirmation of specific markers, such as the expression of immortalization-associated genes or the presence of telomerase activity.
| Cat.No. | Product Name | Price |
|---|---|---|
| LVIM001Z | SV40 Small/Large T Antigen Lentivirus (CMV, Puro) | Inquiry |
| LVIM002Z | SV40 Small/Large T Antigen Lentivirus (EF1α, Puro) | Inquiry |
| LVIM003Z | SV40 Large T Antigen Lentivirus (CMV) | Inquiry |
| LVIM004Z | SV40 Large T Antigen Lentivirus (EF1α, Hygro) | Inquiry |
| LVIM005Z | SV40 Large T Antigen Lentivirus (EF1α) | Inquiry |
| LVIM006Z | Human TERT Lentivirus (CMV, Puro) | Inquiry |
| LVIM007Z | Human TERT Lentivirus (CMV, Hygro) | Inquiry |
| LVIM008Z | Human TERT Lentivirus (EF1α, Hygro) | Inquiry |
| LVIM009Z | Human TERT Lentivirus (CMV) | Inquiry |
| LVIM010Z | Human TERT Lentivirus (EF1α, Puro) | Inquiry |
| LVIM011Z | Human CDK4 Lentivirus (CMV, Puro) | Inquiry |
| LVIM012Z | Human CDK4 Lentivirus (CMV) | Inquiry |
| LVIM013Z | Human HOXB8 Lentivirus (CMV, Puro) | Inquiry |
| LVIM014Z | Human HOXB8 Lentivirus (CMV) | Inquiry |
| LVIM015Z | Human HOXA9 Lentivirus (CMV, Puro) | Inquiry |
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