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Cell Immortalization Lentiviral Particles

Primary cells have a finite number of population doublings in culture conditions. Cell line immortalization is a very important research tool which has been widely used in many laboratories. Through immortalization, primary cells are able to proliferate indefinitely and can thus be cultured for long periods of time. Since an immortal cell line can be grown indefinitely in culture, researchers can save a lot of time and energy from frequent primary cell isolation.

Cell line immortalization can occur naturally or be intentionally induced by introducing exogenous factors. There are several examples of immortalized cell lines, for example, HEK293 which is derived from human fetal cells, NIH3T3 which is a spontaneous mouse fibroblast immortal cell line, Vero which is a spontaneous monkey kidney immortal cell line.

Several factors have been identified that have the ability to induce cell immortalization. These includes simian virus 40 (SV40) T antigen, human TERT, human HOXB8, human HOXA9, human HOXA10, human CDK4, human MYC, mouse Bmi1, HPV E6-E7 etc.

ImmortalizationLV.jpgFigure 1. Schematic diagram of cell immortalization

To establish an immortalized cell line, foreign DNA need to be delivered into target cells. However, many primary cells are difficult to transfect. As an efficient gene delivery tool, replication-deficient lentiviruses are often used in the process of immortalization. Creative Biogene offers a series of lentiviral particles containing genes, including SV40 T, human TERT and HPV-16 E6/E7 etc., that can be used for generating immortalized cell lines.

Browse All Cell Immortalization Lentiviral Particles

For research use only. Not intended for any clinical use.

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