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Ba/F3 Cell Line
| General Information | |
| Organism | Mus musculus (B cell) |
| Cell Line Description | Ba/F3 cell line was originally deposited to our cell bank in 1992 as an IL-3-dependent mouse cell line derived from BALB/c mouse strain and has been distributed since then. Ba/F3, a murine interleukin-3 dependent pro-B cell line, is a popular system for exploring both kinases and their kinase inhibitors, because some protein kinases can render Ba/F3 cells to be independent of IL-3, while their inhibitors antagonize this effect. |
| Strain | BALB/c (B cell); BALB/c (myeloma) |
| Tissue | Lymphocyte |
| Cell Type | Pro B cells |
| Morphology | Mostly single, round (some polymorph) cells in suspension |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | Protein expression, drug screening and biological assays |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n> |
| Images |  |
| Derivation | Spleen cells were fused with NSO myeloma cells. |
| Genes Expressed | Immunoglobulin; monoclonal antibody; against rat type 2B myosin heavy chain |
| Mycoplasma | Negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology | CD11b (+), F4/80 (+) |
| Viruses | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Culture Conditions and Handling | |
| Resuscitation | Thaw the vial as soon as possible upon receipt to insure its viability.
|
| Culture Medium | 90% RPMI 1640 + 10% h.i. FBS + 10 ng/ml mouse IL-3 or 10% vol conditioned medium of WEHI-3B cell line |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 104 viable cells/ml. Keep cell density between 5 X 104 and 5 X 105 viable cells/ml. |
| Split Ratio | 1:10 split |
| Medium Renewal | Add fresh medium every 2 to 3 days |
| Culture Temperature | 37℃ |
| Incubation Condition | 5% CO2 |
| Doubling Time | About 20 hours (DSMZ). |
| Harvest | Maximal density of about 2.5 x 106 cells/ml |
| Storage | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
For research use only. Not intended for any clinical use.