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| General Information | |
| Organism | Mus musculus (B cell) |
| Cell Line Description | Ba/F3 cell line was originally deposited to our cell bank in 1992 as an IL-3-dependent mouse cell line derived from BALB/c mouse strain and has been distributed since then. Ba/F3, a murine interleukin-3 dependent pro-B cell line, is a popular system for exploring both kinases and their kinase inhibitors, because some protein kinases can render Ba/F3 cells to be independent of IL-3, while their inhibitors antagonize this effect. |
| Strain | BALB/c (B cell); BALB/c (myeloma) |
| Tissue | Lymphocyte |
| Cell Type | Pro B cells |
| Morphology | Mostly single, round (some polymorph) cells in suspension |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Applications | Protein expression, drug screening and biological assays |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Culture Conditions and Handling | |
| Resuscitation | Thaw the vial as soon as possible upon receipt to insure its viability.
|
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 104 viable cells/ml. Keep cell density between 5 X 104 and 5 X 105 viable cells/ml. |
| Split Ratio | 1:10 split |
| Medium Renewal | Add fresh medium every 2 to 3 days |
| Culture Temperature | 37℃ |
| Incubation Condition | 5% CO2 |
| Harvest | Maximal density of about 2.5 x 106 cells/ml |
| Storage | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0103 | Human ERBB2 Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0110 | Human p95HER2 Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO01145 | Human ETV6-CSF1R Stable Cell Line - Ba/F3 | Inquiry |
| CSC-RO01148 | Human IL6ST Stable Cell Line - Ba/F3 | Inquiry |
| CSC-RO0116 | Human SLC34A2-ROS1 Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0117 | Human SLC34A2-ROS1/G2032R Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0120 | Human EML4-ALK Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0121 | Human EML4-ALK/L1196M Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0122 | Human EML4-ALK/F1174L Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0123 | Human EML4-ALK/C1156Y Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0124 | Human BCR-ABL Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0125 | Human BCR-ABL-T315I Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0126 | Human EGFR Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0127 | Human EGFR-C797S Stable Cell Line-Ba/F3 | Inquiry |
| CSC-RO0128 | Human EGFR-D770_N771insSVD Stable Cell Line-Ba/F3 | Inquiry |
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