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Jurkat Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionJurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called "JM" (JURKAT and JM are derived from the same patient and are sister clones). On occasion, JM may be a subclone with somewhat divergent features.
TissuePeripheral blood
DiseaseAcute T cell leukemia
GenderMale
Cell TypeT cell leukemia
Product FormatFrozen
Growth ModeNon-adherent
ApplicationsJurkat cell line is a suitable transfection host.
Shipped inDry ice
Storage Temperature−196°C
Culture Conditions and Handling
Thawing Frozen Cells
  1. Thaw the vial by gentle agitation in a 37°C water bath. Thawing should be rapid (about 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations should be carried out under strict aseptic conditions.
  3. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. And spin at approximately 125 x g for 5 to 7 minutes.
  4. Resuspend cell pellet with the complete medium. And dispense into a 25 cm2 or a 75 cm2 culture flask. It is crucial to avoid excessive alkalinity of the medium during recovery of the cells.
  5. Incubate the culture at 37°C in a suitable incubator.
SubculturingCultures can be maintained by replacement of medium or the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Do not allow the cell density to exceed 3 x 106 cells/mL.
Fluid RenewalEvery 2 to 3 days.
Freeze MediumComplete growth medium 95%; DMSO, 5%
Culture Temperature37°C
AtmosphereAir, 95%; carbon dioxide (CO2), 5%
Cat.No.Product NamePrice
CSC-RR0282 GFP Reporter Cell Line - Jurkat Inquiry
CSC-RR0375 Luciferase Reporter Cell Line - Jurkat Inquiry
CSC-RR0387 NFkB Luciferase Reporter Cell Line - Jurkat Inquiry
CSC-RR0389 NFAT Luciferase Reporter Cell Line - Jurkat Inquiry
CSC-RT2315 Human PD1 Knockout Cell Line-Jurkat Inquiry
CSC-RO0179 Cas9 Stable Cell Line - Jurkat Inquiry
RTA-0001 Jurkat-CD16/Dual-Luc Effector Assay Cells Inquiry
CSC-RR0471 FOXP3-Luc Reporter Cell Line-Jurkat Inquiry
CSC-RO0586 Human TIGIT Stable Cell Line - Jurkat Inquiry
CSC-RR0500 NFAT-Luc/PDCD1 Stable Cell Line - Jurkat Inquiry
CSC-RT2699 Human CD47 Knockout Cell Line-Jurkat Inquiry
CSC-RT2085 Human B2M Knockout Cell Line-Jurkat Inquiry
CSC-RR0542 GFP/Luc Reporter Cell Line - Jurkat Inquiry
CSC-RO0709 Monkey TIGIT Stable Cell Line - Jurkat Inquiry
CSC-RR0560 Human LAG3/NFAT-Luc Reporter Cell Line-Jurkat Inquiry
CSC-RR0561 Human CD40/NFkB-Luc Reporter Cell Line-Jurkat Inquiry
CSC-RR0562 Human TIGIT/NFAT-Luc Reporter Cell Line-Jurkat Inquiry
CSC-RO1021 Monkey PDCD1 Stable Cell Line-Jurkat Inquiry
CSC-RO1048 Human CTLA-4 Stable Cell Line-Jurkat Inquiry
CSC-RO1059 Human TNFRSF1B Stable Cell Line-Jurkat Inquiry
CSC-RO1060 Human CD40 Stable Cell Line-Jurkat Inquiry
CSC-RR0565 IL2-Luc Reporter Cell Line - Jurkat Inquiry
CSC-RT2780 Human LRRK2 Knockout Cell Line-Jurkat Inquiry
CSC-RR0599 Human CTLA4/IL2-Luc Reporter Cell Line - Jurkat Inquiry
RTA-0019 Jurkat-CD32/Dual-Luc Effector Assay Cells Inquiry
CSC-RO01139 Human FCGR1A/NFAT-Luc Stable Cell Line - Jurkat Inquiry
CSC-RO01140 Human FCGR2A(131H)/NFAT-Luc Stable Cell Line - Jurkat Inquiry
CSC-RO01141 Human FCGR2A(131R)/NFAT-Luc Stable Cell Line - Jurkat Inquiry
CSC-RO01142 Human FCGR2B/NFAT-Luc Stable Cell Line - Jurkat Inquiry
CSC-RO01143 Human FCGR3A(158V)/NFAT-Luc Stable Cell Line - Jurkat Inquiry
CSC-RO01144 Human FCGR3A(158F)/NFAT-Luc Stable Cell Line - Jurkat Inquiry
CSC-RO01495 OVAL Stable Cell Line - Jurkat Inquiry
CSC-RO01748 GFP/OVAL Stable Cell Line - Jurkat Inquiry
CSC-RO02001 Luc/OVAL Stable Cell Line - Jurkat Inquiry
CSC-RO02254 GFP/Luc/OVAL Stable Cell Line - Jurkat Inquiry
CSC-RR01136 NFκB-Luc/EF1α-mCherry Reporter Cell Line - Jurkat Inquiry

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

* For research use only. Not intended for any clinical use.
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