Jurkat Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	Jurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called "JM" (JURKAT and JM are derived from the same patient and are sister clones). On occasion, JM may be a subclone with somewhat divergent features.
Tissue	Peripheral blood
Disease	Acute T cell leukemia
Gender	Male
Cell Type	T cell leukemia
Product Format	Frozen
Growth Mode	Non-adherent
Applications	Jurkat cell line is a suitable transfection host.
Shipped in	Dry ice
Storage Temperature	−196°C
Culture Conditions and Handling
Thawing Frozen Cells	Thaw the vial by gentle agitation in a 37°C water bath. Thawing should be rapid (about 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations should be carried out under strict aseptic conditions. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. And spin at approximately 125 x g for 5 to 7 minutes. Resuspend cell pellet with the complete medium. And dispense into a 25 cm² or a 75 cm² culture flask. It is crucial to avoid excessive alkalinity of the medium during recovery of the cells. Incubate the culture at 37°C in a suitable incubator.
Subculturing	Cultures can be maintained by replacement of medium or the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10⁵ viable cells/mL. Do not allow the cell density to exceed 3 x 10⁶ cells/mL.
Fluid Renewal	Every 2 to 3 days.
Freeze Medium	Complete growth medium 95%; DMSO, 5%
Culture Temperature	37°C
Atmosphere	Air, 95%; carbon dioxide (CO₂), 5%

The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO01139	Human FCGR1A/NFAT-Luc Stable Cell Line - Jurkat	Inquiry
CSC-RO01140	Human FCGR2A(131H)/NFAT-Luc Stable Cell Line - Jurkat	Inquiry
CSC-RO01141	Human FCGR2A(131R)/NFAT-Luc Stable Cell Line - Jurkat	Inquiry
CSC-RO01142	Human FCGR2B/NFAT-Luc Stable Cell Line - Jurkat	Inquiry
CSC-RO01143	Human FCGR3A(158V)/NFAT-Luc Stable Cell Line - Jurkat	Inquiry
CSC-RO01144	Human FCGR3A(158F)/NFAT-Luc Stable Cell Line - Jurkat	Inquiry
CSC-RO0179	Cas9 Stable Cell Line - Jurkat	Inquiry
CSC-RO0586	Human TIGIT Stable Cell Line - Jurkat	Inquiry
CSC-RO0709	Monkey TIGIT Stable Cell Line - Jurkat	Inquiry
CSC-RO1021	Monkey PDCD1 Stable Cell Line-Jurkat	Inquiry
CSC-RO1048	Human CTLA-4 Stable Cell Line-Jurkat	Inquiry
CSC-RO1059	Human TNFRSF1B Stable Cell Line-Jurkat	Inquiry
CSC-RO1060	Human CD40 Stable Cell Line-Jurkat	Inquiry
CSC-RR0282	GFP Reporter Cell Line - Jurkat	Inquiry
CSC-RR0375	Luciferase Reporter Cell Line - Jurkat	Inquiry

* For research use only. Not intended for any clinical use.

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