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Jurkat Cell Line


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Jurkat Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionJurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called "JM" (JURKAT and JM are derived from the same patient and are sister clones). On occasion, JM may be a subclone with somewhat divergent features.
TissuePeripheral blood
DiseaseAcute T cell leukemia
GenderMale
Cell TypeT cell leukemia
Product FormatFrozen
Growth ModeNon-adherent
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsJurkat cell line is a suitable transfection host.
Shipped inDry ice
Storage Temperature−196°C
Characteristics
KaryotypeThis is a pseudodiploid human cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%. The karyotype is 46, XY, -2, -18, del(2) (p21p23), del(18) (p11.2). Most cells have normal X and Y chromosomes.
Images
MycoplasmaContamination is eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, PCR assays and RNA hybridization.
ImmunologyCD2 +, CD3 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, TCR-α/β +, TCR-γ/δ -;
FingerprintMultiplex PCR of minisatellite markers reveals a unique DNA profile.
VirusesELISA: reverse transcriptase negative;
PCR: HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, EBV -, MLV -, SMRV -
CommentsJurkat cells are an immortalized line of T lymphocyte cells. And they are used to study acute T cell leukemia, T cell signaling, and the expression of a number of chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science due to their ability to produce interleukin 2. However, their primary use is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.
Culture Conditions and Handling
Thawing Frozen Cells
  1. Thaw the vial by gentle agitation in a 37°C water bath. Thawing should be rapid (about 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations should be carried out under strict aseptic conditions.
  3. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium. And spin at approximately 125 x g for 5 to 7 minutes.
  4. Resuspend cell pellet with the complete medium. And dispense into a 25 cm2 or a 75 cm2 culture flask. It is crucial to avoid excessive alkalinity of the medium during recovery of the cells.
  5. Incubate the culture at 37°C in a suitable incubator.
Culture Medium90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine
SubculturingCultures can be maintained by replacement of medium or the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Do not allow the cell density to exceed 3 x 106 cells/mL.
Fluid RenewalEvery 2 to 3 days.
Freeze MediumComplete growth medium 95%; DMSO, 5%
Culture Temperature37°C
AtmosphereAir, 95%; carbon dioxide (CO2), 5%

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