Jurkat Cell Line
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Jurkat Cell Line
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Jurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called "JM" (JURKAT and JM are derived from the same patient and are sister clones). On occasion, JM may be a subclone with somewhat divergent features. |
| Tissue | Peripheral blood |
| Disease | Acute T cell leukemia |
| Gender | Male |
| Cell Type | T cell leukemia |
| Product Format | Frozen |
| Growth Mode | Non-adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | Jurkat cell line is a suitable transfection host. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%. The karyotype is 46, XY, -2, -18, del(2) (p21p23), del(18) (p11.2). Most cells have normal X and Y chromosomes. |
| Images |  |
| Mycoplasma | Contamination is eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, PCR assays and RNA hybridization. |
| Immunology | CD2 +, CD3 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, TCR-α/β +, TCR-γ/δ -; |
| Fingerprint | Multiplex PCR of minisatellite markers reveals a unique DNA profile. |
| Viruses | ELISA: reverse transcriptase negative; PCR: HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, EBV -, MLV -, SMRV - |
| Comments | Jurkat cells are an immortalized line of T lymphocyte cells. And they are used to study acute T cell leukemia, T cell signaling, and the expression of a number of chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science due to their ability to produce interleukin 2. However, their primary use is to determine the mechanism of differential susceptibility of cancers to drugs and radiation. |
| Culture Conditions and Handling | |
| Thawing Frozen Cells |
|
| Culture Medium | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculturing | Cultures can be maintained by replacement of medium or the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Do not allow the cell density to exceed 3 x 106 cells/mL. |
| Fluid Renewal | Every 2 to 3 days. |
| Freeze Medium | Complete growth medium 95%; DMSO, 5% |
| Culture Temperature | 37°C |
| Atmosphere | Air, 95%; carbon dioxide (CO2), 5% |
| Cat.No. | Product Name | Price |
|---|---|---|
| RTA-0001 | Jurkat-CD16/Dual-Luc Effector Assay Cells | Inquriy |
| CSC-RR0282 | GFP Reporter Cell Line - Jurkat | Inquriy |
| CSC-RR0375 | Luciferase Reporter Cell Line - Jurkat | Inquriy |
| CSC-RR0387 | NFkB Luciferase Reporter Cell Line - Jurkat | Inquriy |
| CSC-RR0389 | NFAT Luciferase Reporter Cell Line - Jurkat | Inquriy |
| CSC-RT2315 | Human PD1 Knockout Cell Line-Jurkat | Inquriy |
| CSC-RO0179 | Cas9 Stable Cell Line-Jurkat | Inquriy |
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