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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The 786-O cell line, derived from a primary tumor from a 58-year-old male patient with renal adenocarcinoma, provides an excellent model for studying cell signaling and molecular biology in the context of renal cell carcinoma (RCC). These cells exhibit an epithelial-like morphology and grow as an adherent monolayer, mimicking the characteristics of renal epithelial cells. These cells possess microvilli and desmosomes and have been successfully cultured in soft agar media. 786-O cells also produce a peptide that is structurally similar to PTH (parathyroid hormone), which is also found in breast and lung cancers. This peptide has an N-terminal sequence similar to that of PTH and is also comparable in activity. Its molecular weight reaches 6000 Daltons. The 786-O cell line is known to be highly invasive and has been used to study the effects of various drugs and treatments on renal cell carcinoma cells. |
| Tissue | Kidney |
| Disease | Adenocarcinoma; Renal Cell |
| Morphology | Epithelial |
| Gender | Male |
| Age | 58 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Studying the molecular mechanisms of renal cell carcinoma (RCC) development and progression. 2. Screening and testing potential anti-cancer drugs for the treatment of RCC. 3. Investigating the role of specific genes, signaling pathways, and biomarkers in RCC. 4. Studying the interaction between RCC cells and the tumor microenvironment. 5. Developing and testing new therapeutic strategies for RCC, such as targeted therapies and immunotherapies. 6. Understanding drug resistance mechanisms in RCC. 7. Studying the metastatic potential of RCC cells. 8. Investigating the effects of various treatments on RCC cell viability, proliferation, and migration. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | A key feature of the 786-O cell line is the lack of the von Hippel-Lindau (VHL) tumor suppressor gene. In renal cell carcinoma, the VHL gene is frequently mutated or deleted, leading to dysregulation of various cellular processes, including angiogenesis and metabolism. The lack of VHL expression in 786-O cells reflects common mutations in RCC and provides an opportunity to explore downstream signaling pathways affected by VHL. |
| Characteristics | |
| Karyotype | Hypertriploid; Y was present in 60% the cells examined. |
| Genes Expressed | Parathyroid hormone (PTH) like peptide |
| Tumorigenic | Yes |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Rinse the cell layer briefly with DPBS solution to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | RPMI-1640 +10% FBS +1% P/S |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃ |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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