Rice Endosperm-Specific Expression System

Creative Biogene has established a high-yield system for plant seeds-based, eukaryotic expression and purification of recombinant proteins that are free from human, animal, or microbial contaminants. We utilize rice endosperm-specific expression system to offer recombination proteins and polypeptides expression service. Based on fully mature platform, we provide protein and peptide purification process development services.

Rice seeds are an ideal production platform for molecular farming, as recombinant proteins can accumulate at high levels in the rice endosperm through established production systems. Rice seed bioreactors offer many advantages for producing recombinant proteins, including their stable accumulation in a small volume and the easy long-term storage of protein avoiding degradation at ambient temperature. These make the seed-based platform suitable for many recombinant proteins produced on a large scale. Up to now, rice endosperm cell-based expression system using RAmy3D promoter has applied in high-level expression of many pharmaceutical proteins, such as rAAT, hGM-CSF, interleukin-12, human serum albumin.

Recombination protein expression services

Phase 1: Generation of genetic modified crops

Codon optimization, gene synthesis, selection of endosperm-specific promoter, and vector construction
Agrobacterium-mediated rice transformation
Molecular characterization
Screening the transgenic line of high-level expression

Phase 2: Lab scale

Screening the homozygous lines that possess stable inheritance
Bioactivity analysis of recombinant protein
Microgram scale recombination protein with 90-95% purity

Phase 3: Pilot-scale

Milligram or gram scale recombination protein with 95% purity
Biosafety analysis of GMOs (genetic modified crops)

Phase 4: Large-scale

Large-scale recombinant protein production

Benefits

High product yields: ten-fold higher yields compared to other plant expression systems.
Safe background: orally available products produced in a plant that is safe for oral delivery.
Eukaryotic expression system: rice can manufacture complex biologics that are difficult to make in other hosts, including proteins requiring post-translational modification.
Flexibility: ability to manufacture multi-subunit molecules, monoclonal antibodies, fusion proteins, and enzymes.
Purity: products that are inherently free of animal, human, or microbial by-products, making them ideal for therapeutic use or for use in sensitive and exacting biotechnology applications.
Cost-effectiveness: suitable for cGMP manufacturing with dramatically lower cost of production.
Scalability: recombinant protein yields from kilograms to hundreds of metric tons.
Storability: mature rice grain can be stored without product degradation.
Biosecurity: rice is self-pollinating, maintaining genetic purity, with a chain of custody trackable from target gene to final biologic.

Creative Biogene's scientists have been able to harness the natural life cycle and growth of rice as a background in which to manufacture highly pure recombinant proteins. Our service can be tailored according to your specialized requirements (like codon optimization, tag selection, endosperm-specific promoter selection, purification process.). Our expertise guarantees high protein quality, short turnaround time, and the best price in the market.

* For research use only. Not intended for any clinical use.

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