Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | BJ is a human fibroblast cell line derived from the foreskin of a normal male newborn. The cell line was established in 1963 by Gey and colleagues and has since been widely used in biomedical research, including studies of aging, cancer, and viral infection. BJ cells have a diploid karyotype and can be cultured adherently in vitro. They are a valuable tool for studying cellular senescence and aging-related changes, as they have a limited lifespan in culture and eventually enter a state of replicative senescence after a certain number of cell divisions. The human cell lines BJ and HaCaT are often used together in dermatological studies, as they are complementary in representing key aspects of skin physiology. BJ cells are also used as a model system to study the effects of viral infection on host cells. They have been infected with a variety of viruses, including human papillomavirus (HPV), adenovirus, and herpes simplex virus (HSV), to study viral replication and pathogenesis. |
| Tissue | Skin; Foreskin |
| Disease | Normal |
| Morphology | Fibroblast |
| Gender | Male |
| Age | Neonate |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Telomere biology and aging studies 3. Drug screening and development 4. Toxicology studies 5. Skin disease research 6. Wound healing and regenerative medicine |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The BJ cell line has a long lifespan in comparison with other normal human fibroblast cell lines. |
| Characteristics | |
| Karyotype | BJ cells maintain a normal diploid karyotype. However, beyond a certain population doubling, abnormal karyotypes indicative of genetic alterations may appear. |
| Tumorigenic | No |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old medium from adhered cells and wash with PBS without calcium and magnesium. For T25 flasks, use 3-5 ml PBS and for T75 flasks, use 5-10 ml. 2. Then completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to incubate at room temperature for 8-10 minutes to allow them to detach. 4. After incubation, gently mix the cells with 10 ml medium to resuspend, then centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells with fresh medium, and transfer them to a new flask already containing fresh medium. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:2 to 1:9 is recommended. |
| Culture Medium | EMEM + 10% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO01168 | Cas9 Stable Cell Line - BJ | Inquiry |
| CSC-RR00634 | GFP Reporter Cell Line - BJ | Inquiry |
| CSC-RR00665 | Luciferase Reporter Cell Line - BJ | Inquiry |
Copyright © Creative Biogene. All rights reserved.