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Bridged Nucleic Acids (BNA), also known as "bridged nucleic acids" or "locked nucleic acids," are a class of nucleic acid analogs designed based on the conformational behavior of natural nucleic acids. Traditional nucleic acids, DNA and RNA, exhibit considerable conformational flexibility at the 2' and 4' positions of the sugar ring. While this flexibility facilitates molecular movement, it hinders stable double-strand formation, resulting in suboptimal thermodynamic stability and vulnerability to nuclease degradation. BNA addresses this by introducing a bridge structure on the ribose ring—such as a 2'-O,4'-C or 2'-O,4'-aminoethylene bridge—that significantly restricts conformational freedom. This structural constraint enhances hybridization affinity and thermal stability when binding to complementary DNA or RNA strands, while also providing strong nuclease resistance.
Figure 1. Structure of BNA.
To date, more than ten types of BNA have been developed, including first-generation LNAs and second-generation BNANC. Different types vary in hybridization affinity, nuclease stability, and off-target discrimination. Third-generation BNANC, in particular, exhibits high RNA-selective binding, excellent mismatch discrimination, and superior nuclease resistance compared to traditional phosphorothioate-modified oligonucleotides.
Creative Biogene is a leading provider of molecular biology and nucleic acid synthesis services. With extensive industry experience, a team of recognized scientists, and advanced synthesis platforms, we offer comprehensive, one-stop BNA oligonucleotide services—from design and synthesis to quality control—for research customers worldwide. Our dedicated IntegrateRNA platform specializes in high-purity BNA oligonucleotide customization, accommodating various structural modifications, scales, and quality requirements suitable for laboratory research and preclinical development.
We provide end-to-end services starting from initial sequence design to final delivery. Our experienced technical team collaborates closely with clients to incorporate BNA monomers into DNA/RNA sequences, optimizing thermodynamic properties and binding specificity. Using standard phosphoramidite chemistry and solid-phase synthesis platforms, we synthesize BNA-DNA and BNA-RNA hybrid oligonucleotides with flexible design options for BNA placement and ratio.
Post-synthesis, products can be deprotected, desalted, and purified via HPLC based on client needs. Quality validation is performed using MALDI-TOF or ESI mass spectrometry to ensure product reliability and suitability for scientific research. We support a wide range of modifications, including fluorescent labeling, biotinylation, amino-linkers, thiol groups, and phosphorylation to meet diverse applications in probes, tags, and functional molecules.
We accommodate a wide range of production scales—from small-scale research experiments (micromole quantities), pilot batches, to large-scale in vivo model or preclinical research quantities up to milligrams or grams. Creative Biogene offers synthesis scales starting from 0.05 µmol and above, with full quality control documentation including mass spectrometry data, purity reports, and technical support to ensure smooth project progression.
Our BNA oligonucleotides have been extensively applied in molecular biology research and biomedical development, including but not limited to:
Notable case studies include BNANC-DNA gapmers reversing splicing defects in myotonic dystrophy model cells with low toxicity, suppression of antibiotic resistance genes in bacterial strains, and enhanced CRISPR-Cas9 specificity by incorporating BNANC substitutions in guide RNAs, underscoring the potential of BNA oligonucleotides in gene modulation and editing enhancement.
Creative Biogene's BNA synthesis strictly follows standard operating procedures (SOPs) to ensure high-quality production, including:
1Contact our technical team with your target sequences and experimental requirements.
2Discuss BNA types (e.g., BNANC, BNAcoc), insertion positions, and desired modifications.
3Finalize scale, purification methods, and delivery timelines.
4Upon contract signing, we proceed with synthesis → purification → QC → delivery.
5Products come with detailed mass spec and purity reports along with usage recommendations.
Creative Biogene's BNA oligonucleotide synthesis service combines the latest BNA structural innovations (such as BNANC and BNAcoc), mature synthesis platforms, and rigorous quality control to deliver products with high affinity, nuclease resistance, precise mismatch recognition, and diverse modification options. These oligonucleotides are broadly applicable in antisense, RNAi, SNP detection, FISH, PCR, and miRNA/ncRNA functional studies. Whether you are in basic research or preclinical development, Creative Biogene's BNA service offers reliable, high-quality, and customizable oligonucleotide solutions.
For detailed pricing, delivery times, or further technical inquiries, please visit the Creative Biogene website, our IntegrateRNA platform, or contact our technical support team directly.
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