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Recombinant adenovirus particles are versatile for genetic material delivery, featuring large gene packaging, high-titer purification, efficient transfer into diverse mammalian cells, replication defects, and absence of genome integration. These properties make adenoviral vectors vital for transgene expression and genome editing. Studies have demonstrated the effectiveness of recombinase-expressing adenoviruses in manipulating genes in various host cells. Creative Biogene offers highly qualified recombinase-expressing adenovirus particles that are suitable for both cell experiments and animal injections.
Advantages of Our Recombinase Adenovirus Particles
Recombinase Adenovirus Particles Product List
The widespread adoption of the Cre-lox system and GFP-expressing adenoviruses for precise gene editing and recombination studies has become a prominent technique in the scientific community. These versatile adenoviruses play a crucial role in genetic manipulation experiments across various cell types, particularly advancing applications in targeted gene editing and monitoring within human cells. Our products offer the following capabilities:
Case Study 1
CTNNB1, a proto-oncogene encoding β-catenin, exhibits frequent gain-of-function mutations in hepatic neoplasms. The researchers unveil the significance and pathological mechanism of CTNNB1 mutations in hepatocarcinogenesis. Inhibiting the β-catenin/AKT2-stimulated pyrimidine synthesis axis selectively suppresses β-catenin mutant cell proliferation and tumor formation, suggesting a druggable vulnerability for β-catenin mutant liver cancer.
Figure 1. Brequinar (BRQ) inhibited liver tumor initiation and progression in HBV; β-cateninlox(ex3)/+ mice injected with Cre-adenoviruses demonstrated promising therapeutic potential, with no significant impact on body weight and organs, except for an enlarged spleen. Targeting AKT2-potentiated pyrimidine synthesis emerges as a promising strategy for β-catenin mutant liver cancer. (Liu F, et al., 2022)
Case Study 2
Reactive oxygen species (ROS) damage cellular structures and cause oxidative stress, but they also function as signaling molecules that regulate biological and pathological processes. The researchers found that mitochondria-targeted antioxidants MitoQ and MitoTEMPO had no impact on tumor progression in BRAF-induced melanoma and KRAS-induced lung cancer mouse models. Additionally, these antioxidants did not affect DNA damage levels or proliferation in human melanoma and lung cancer cell lines. The glycolytic rates and respiration were altered by MitoQ and its control substance dTPP in melanoma cells, while only dTPP affected lung cancer cells.
Figure 2. Six- to eight-week-old Kras2LSL/+ mice inhaled a Cre-adenovirus (5 × 107 pfu), followed by randomized treatments with MitoQ, dTPP, MitoTEMPO, or PBS. No significant effects on body weight or water intake were observed. The mouse experiments were ethically approved. (Le Gal K, et al., 2021)
Q: Which recombinase enzymes are commonly delivered by these particles, and what are their roles?
A: Commonly delivered recombinase enzymes include Cre and Flp, each with distinct recognition sites. Cre mediates recombination at loxP sites, while Flp acts at FRT sites.
Q: How are Recombinase Adenovirus Particles employed in genetic engineering experiments?
A: Researchers introduce Recombinase Adenovirus Particles into cells containing specific DNA sequences flanked by recombination sites (e.g., loxP or FRT), leading to targeted recombination and DNA modification.
Q: What are the advantages of using Recombinase Adenovirus Particles for site-specific recombination?
A: Recombinase Adenovirus Particles enable precise and controllable site-specific recombination, ensuring accuracy in genetic modifications. Researchers can use these particles for a variety of applications, offering versatility in genetic engineering experiments.
Q: In what research areas or experimental setups are Recombinase Adenovirus Particles commonly utilized?
A: These particles are frequently used in studies involving conditional gene knockout, lineage tracing, and the creation of genetically modified animal models, contributing to advancements in various biological fields.
| Cat.No. | Product Name | Price |
|---|---|---|
| AD00294Z | Human Cre-GFP adenoviral particles | Inquiry |
| AD00299Z | Cre adenoviral particles | Inquiry |
| AD00300Z | Cre RSV GFP adenoviral particles | Inquiry |
| AD18659Z | Ad5/RGD-Cre Chimeric Adenovirus | Inquiry |
| AD18660Z | Ad5/F35-Cre Chimeric Adenovirus | Inquiry |
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