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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MHCC97-L is a human hepatocellular carcinoma (HCC) cell line characterized by low metastatic potential. This cell line was established by the Liver Cancer Institute at Fudan University in China to serve as a biological counterpart to the highly metastatic cell line, MHCC97-H. Both cell lines are derived from the same parental cell line, MHCC97, which was originally obtained from a 39-year-old male patient with HCC and a history of hepatitis B virus (HBV) infection. MHCC97-L exhibits an epithelial morphology and serves as a critical comparative model for investigating the molecular mechanisms underlying tumor metastasis. In the field of experimental oncology, this cell line is widely utilized to identify differential gene expression between highly and lowly metastatic variants, to study the process of epithelial-mesenchymal transition (EMT), and to evaluate the anti-metastatic efficacy of novel drugs and gene therapies. |
| Tissue | Liver |
| Disease | Hepatocellular Carcinoma (HCC) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 39 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Comparative research on liver cancer metastasis mechanisms 2. Study of tumor cell invasion and migration signaling pathways 3. Identification of molecular markers for low vs. high metastatic potential 4. Screening for anti-angiogenic and anti-tumor therapeutic agents 5. Development of orthotopic and subcutaneous HCC xenograft models |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Metastatic Potential | Low (compared to MHCC97-H and HCCLM3) |
| Genetic Profile | HBV-DNA positive; Alpha-fetoprotein (AFP) positive; TP53 mutation |
| Karyotype | Aneuploid; hypertriploid |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically requiring 3 to 8 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00799 | Luciferase Reporter Cell Line - MHCC97-L | Inquiry |
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