Fibroblast activation protein (FAP) is a type II cell surface serine protease that belongs to the prolyl oligopeptidase family. It is highly expressed in activated fibroblasts associated with various pathological conditions, such as cancer, fibrosis, and wound healing. In the tumor microenvironment, FAP - expressing cancer - associated fibroblasts (CAFs) play a crucial role in extracellular matrix (ECM) remodeling, tumor progression, immune evasion, fibrosis, and metastasis. Due to its selective expression in diseased tissues, FAP has emerged as a promising therapeutic target and diagnostic marker.

FAP has been found to be overexpressed in many types of cancers, including breast, lung, pancreatic, and colorectal cancer. It promotes tumor progression by remodeling the extracellular matrix, enhancing angiogenesis, and modulating the immune response. For example, in pancreatic cancer, FAP - positive fibroblasts help create a permissive environment for tumor cell growth and survival.

In fibrotic diseases like idiopathic pulmonary fibrosis and liver fibrosis, FAP - expressing myofibroblasts are key players in excessive extracellular matrix deposition, leading to tissue scarring and loss of organ function.

Current research is focused on developing FAP - targeted therapies. Monoclonal antibodies against FAP are being investigated for cancer immunotherapy, aiming to specifically target and kill FAP - positive cells in the tumor microenvironment. Additionally, small molecule inhibitors of FAP are being developed to block its proteolytic activity, which may have potential in treating both cancer and fibrosis.

Creative Biogene has over 15 years of experience in stable cell line construction. We have a team of highly skilled scientists who are proficient in various gene - delivery techniques, including plasmid transfection, electroporation and lentivirus transduction. Our state - of - the - art facilities allow us to perform high - throughput cell line generation and screening, ensuring the quality and consistency of our products. With years of expertise in stable cell line development, Creative Biogene has developed multiple ready-to-use FAP-overexpressing cell models to accelerate your research. Choose our FAP stable cell lines for reliable, high - quality research tools that can accelerate your studies in cancer, fibrosis, and other FAP - related fields.

Product List

Product Features and Advantages

• Diverse Host Cell Options: We offer both human and mouse FAP/Fap stable cell lines, with a variety of host cell types such as HEK293T, KB, HT1080, HPAF-II for human; and NIH/3T3, CT26, B16F10, MC38, MB49 for mouse, allowing researchers to choose the most suitable system for their experiments.
• High Expression Levels: Our stable cell lines are engineered to have high levels of human or mouse FAP/Fap expression, which can be easily detected by QPCR and/or flow cytometry, providing clear signals for research.
• Comprehensive Quality Control: Each batch of our cell lines undergoes strict quality control. We use both PCR and Lonza kit methods to detect mycoplasma contamination, ensuring the purity and integrity of the cells.
• Fast Delivery: For our in - stock products, we guarantee a quick delivery cycle, reducing waiting time for researchers and enabling them to start their experiments promptly.
• Customization Services: In addition to our standard products, we also offer customization services. If you have specific requirements for the cell line, such as a specific cell type, our team can work with you to develop a tailored solution.

Product Applications

FAP overexpressing stable cell lines have several applications in various fields, such as cancer research, tissue engineering, and drug development:

1. Cancer Research

1) Modeling Tumor Microenvironment: FAP is highly expressed in cancer - associated fibroblasts in many tumors. Stable cell lines overexpressing FAP can be used to create in vitro and in vivo models that mimic the tumor microenvironment. This helps researchers study the role of FAP - expressing fibroblasts in tumor growth, invasion, and metastasis.

2) Target Validation: They serve as valuable tools for validating FAP as a therapeutic target. By comparing the behavior of cancer cells co - cultured with FAP - overexpressing cells versus normal cells, the specific effects of FAP on cancer cell proliferation, survival, and angiogenesis can be determined. This provides crucial evidence for the development of FAP - targeted cancer therapies.

3) Drug Screening: Stable cell lines can be used in high - throughput drug screening assays to identify compounds that specifically target FAP or its downstream signaling pathways. These cell lines allow for the rapid and efficient evaluation of the efficacy and toxicity of potential anti - cancer drugs, facilitating the discovery of novel therapeutic agents.

2. Tissue Engineering

Wound Healing Studies: FAP - expressing cells are involved in the process of wound healing and tissue repair. Overexpressing FAP in stable cell lines can help researchers understand the mechanisms underlying fibroblast activation and extracellular matrix remodeling during wound healing. This knowledge can be applied to develop new strategies for promoting efficient wound closure and tissue regeneration.

3. Immunology and Inflammation Research

1) Inflammatory Response Modeling: FAP is also expressed in activated fibroblasts during inflammatory responses. Stable cell lines overexpressing FAP can be used to study the role of FAP - positive fibroblasts in the recruitment and activation of immune cells, as well as the production of inflammatory cytokines and chemokines. This contributes to a better understanding of the immunological mechanisms underlying inflammatory diseases and the development of targeted anti - inflammatory therapies.

2) Vaccine Development: FAP - overexpressing cell lines can be used to produce FAP - specific antigens for vaccine development. These antigens can be used to stimulate an immune response against FAP - expressing cells, which may have potential applications in treating diseases where FAP - positive cells play a pathogenic role, such as cancer and certain fibrotic disorders.

4. Drug Development

1) Pharmacokinetic and Toxicity Studies: In pre - clinical drug development, FAP - overexpressing stable cell lines can be used to study the pharmacokinetics and toxicity of FAP - targeted drugs. By analyzing the uptake, metabolism, and excretion of drugs in these cell lines, researchers can optimize drug dosing and formulation to improve the safety and efficacy of FAP - targeted therapies.

2) Evaluating Drug Efficacy: These cell lines provide a relevant model system for evaluating the efficacy of FAP - targeted drugs in vitro before conducting in vivo animal studies and clinical trials. This allows for the early identification and optimization of drug candidates, reducing the cost and time required for drug development.

Product Representative Data

1. QPCR Results: QPCR analysis shows a significant overexpression level in our FAP/Fap stable cell lines.

2. Flow Cytometry Data: Flow cytometry results demonstrate a high percentage of FAP - positive cells. High percent of FAP cell population shows strong fluorescence when stained with an anti - FAP antibody, indicating high - level cell surface expression of FAP.

3. Mycoplasma Detection: We use two methods, PCR plus MycoAlert kit, monitoring mycoplasma contamination through the whole process of the stable cell line generation.

4. Cell Morphology: Cell images under microscopy show the typical morphological characteristics of our FAP stable cell lines.