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| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | RBL-2H3 is a histamine-releasing cell line derived from rat basophilic leukemia. It is widely considered the gold standard model for studying IgE-mediated degranulation, mast cell activation, and immediate-type hypersensitivity (allergy) pathways. These cells express a high-affinity IgE receptor (FcεRI) on their surface. When an antigen cross-links with the surface-bound IgE, RBL-2H3 cells rapidly undergo exocytosis, releasing histamine, β-hexosaminease, and various cytokines, among other inflammatory mediators. Due to its stable and reproducible response, this cell line is widely used in pharmacology and immunology for screening anti-allergic compounds and studying signal transduction mechanisms. |
| Tissue | Peripheral blood |
| Disease | Basophilic Leukemia |
| Morphology | Fibroblastic; Epithelial-like |
| Gender | Not specified |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Research on IgE-mediated mast cell degranulation and allergic reactions 2. Screening of antihistamines and anti-allergy drugs 3. Research on FcεRI receptor signaling and membrane transport 4. Research on calcium signaling and inflammatory mediator release 5. Development of biosensors for detecting environmental allergens |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid; highly variable chromosomal number |
| Tumorigenic | Yes, in syngeneic rats |
| Expression Markers | High-affinity IgE receptor (FcεRI); rat mast cell protease (RMCP) |
| Secretory Products | Histamine; β-hexosaminease; leukotrienes; cytokines (e.g., TNF-α) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | MEM or DMEM supplemented with 15% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00824 | Luciferase Reporter Cell Line - RBL-2H3 | Inquiry |
| CSC-RR00825 | GFP Reporter Cell Line - RBL-2H3 | Inquiry |
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