COS-7 Cell Line

Brief Introdution
Product List

General Information
Organism	Cercopithecus aethiops
Cell Line Description	COS-7 cells are a fibroblast-like cell line derived from African green monkey kidney tissue. The COS-7 cell line was established by Professor Yakov Gluzman in 1981. It is derived from a CV-1 African green monkey fibroblast cell line by transformation with a mutant strain of Simian Virus 40 (SV40) that codes for the wild-type T-antigen. The traditional method achieves transfection efficiencies of up to 80% in COS-7 cells, demonstrating its ease of genetic manipulation. COS-7 cells are capable of housing and replicating large plasmids, resulting in high yields of desired recombinant proteins, making them a valuable resource for a variety of applications. COS-7 cells exhibit strong sensitivity to a variety of viruses, making them an excellent model for virology research, including virus-host interaction studies, viral life cycle elucidation, and antiviral drug testing.
Tissue	Kidney
Morphology	Fibroblast
Gender	Male
Age	Adult
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Studying melanoma biology 2. Drug screening 3. Gene expression studies 4. Signaling pathway analysis 5. High-throughput screening 6. Toxicology studies
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	Transformation of CV-1 to create the COS-7 cell line results in the expression of the SV40 T antigen, which retains this antigen and is sensitive to SV40, which grows lytically in kidney cells. A209 viruses, like SV40 mutants, are also allowed to replicate at 40°C as long as the mutations (i.e., insertions, deletions, mutations) are located in early regions of the viral genome. Therefore, COS is the abbreviation of CV-1 in Origin, carrying SV40.
Characteristics
Genes Expressed	T antigen
Virus Susceptibility	SV40 (lytic growth), SV40 tsA209 at 40°C, SV40 mutants with deletions in the early region
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37°C. NOTE: The volumes used in this protocol are for a 75 cm² flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:4 to 1:8 is recommended.
Culture Medium	90% DMEM + 10% h.i. FBS
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37°C
Cryopreservation	Frozen with 70% medium, 20% FBS, 10% DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO0290	Human ACE2 Stable Cell Line - COS-7	Inquiry
CSC-RR0281	GFP Reporter Cell Line - COS-7	Inquiry
CSC-RR0396	HIF Luciferase Reporter Cell Line - Cos-7	Inquiry

* For research use only. Not intended for any clinical use.

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