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CT26 Cell Line
| General Information | |
| Organism | Mus musculus, mouse |
| Synonyms | CT-26; CT 26; CT-26 WT |
| Cell Line Description | CT26 is a murine colorectal carcinoma cell line which is from a BALB/c mouse. The cell is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. These cells are adherent and have a fibroblast morphology. They will form tumors and metastases post implantation into syngenic BALB/c mice or immunocompromised mice. |
| Tissue | Colon |
| Disease | Mouse colon adenocarcinoma |
| Strain | BALB/c |
| Cell Type | Fibroblast |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | CT26 cells are suitable for in vitro and in vivo experimentation. These syngenic BALB/c mice and immunocompromised mice can be used for life studies, and will form tumors following implantation of the cells. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Images |  CT26.WT-iRFP-Neo is a polyclonal population of the mouse colorectal carcinoma cell line CT26.WT. |
| Tumorigenic | Yes |
| Distributibility | Yes |
| Effects | Yes, in BALB/c mice. Mice are inoculated, subcutaneously, developed lethal tumors at 80% frequency with 103 cells and at 100% with 104 cells. Pulmonary metastases develop when mice are inoculated, intravenously, with 104 cells. |
| Subclone CT26.CL25 | CT26.WT is stably transduced with the retroviral vector LXSN that contains the lacZ gene encoding the model tumor associated antigen (TAA), β-galactosidase (β-gal) to obtain the lethal subclone CT26.CL25. The growth rate and lethality of CT26.CL25 and CT26.WT is virtually identical despite the expression by CT26.CL25 of the model TAA, β-galactosidase, in normal mice. |
| Comments | The cells are assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests are negative. |
| Culture Conditions and Handling | |
| Culture Medium | Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS), 10% |
| Subculturing |
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| Subcultivation Ratio | The ratio of 1:4 to 1:10 is recommended. |
| Fluid Renewal | Every 2 to 3 days. |
| Freeze Medium | Complete growth medium 95%; DMSO, 5% |
| Culture Temperature | 37°C |
| Atmosphere | Carbon dioxide (CO2), 5%; air, 95% |
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For research use only. Not intended for any clinical use.