CT26 Cell Line

Brief Introdution
Product List

General Information
Organism	Mus musculus, mouse
Synonyms	CT-26; CT 26; CT-26 WT
Cell Line Description	CT26 is a murine colorectal carcinoma cell line which is from a BALB/c mouse. The cell is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. These cells are adherent and have a fibroblast morphology. They will form tumors and metastases post implantation into syngenic BALB/c mice or immunocompromised mice.
Tissue	Colon
Disease	Mouse colon adenocarcinoma
Strain	BALB/c
Cell Type	Fibroblast
Product Format	Frozen
Growth Mode	Adherent
Applications	CT26 cells are suitable for in vitro and in vivo experimentation. These syngenic BALB/c mice and immunocompromised mice can be used for life studies, and will form tumors following implantation of the cells.
Shipped in	Dry ice
Storage Temperature	−196°C
Culture Conditions and Handling
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: In order to avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersal. Add 6.0-8.0 mL of complete growth medium and then aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to the new culture vessels. Incubate cultures at 37°C.
Subcultivation Ratio	The ratio of 1:4 to 1:10 is recommended.
Fluid Renewal	Every 2 to 3 days.
Freeze Medium	Complete growth medium 95%; DMSO, 5%
Culture Temperature	37°C
Atmosphere	Carbon dioxide (CO₂), 5%; air, 95%

The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

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* For research use only. Not intended for any clinical use.

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