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CT26 Cell Line

General Information
OrganismMus musculus, mouse
SynonymsCT-26; CT 26; CT-26 WT
Cell Line DescriptionCT26 is a murine colorectal carcinoma cell line which is from a BALB/c mouse. The cell is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. These cells are adherent and have a fibroblast morphology. They will form tumors and metastases post implantation into syngenic BALB/c mice or immunocompromised mice.
DiseaseMouse colon adenocarcinoma
Cell TypeFibroblast
Product FormatFrozen
Growth ModeAdherent
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsCT26 cells are suitable for in vitro and in vivo experimentation. These syngenic BALB/c mice and immunocompromised mice can be used for life studies, and will form tumors following implantation of the cells.
Shipped inDry ice
Storage Temperature−196°C
Images CT26.WT-iRFP-Neo is a polyclonal population of the mouse colorectal carcinoma cell line CT26.WT.
EffectsYes, in BALB/c mice. Mice are inoculated, subcutaneously, developed lethal tumors at 80% frequency with 103 cells and at 100% with 104 cells. Pulmonary metastases develop when mice are inoculated, intravenously, with 104 cells.
Subclone CT26.CL25CT26.WT is stably transduced with the retroviral vector LXSN that contains the lacZ gene encoding the model tumor associated antigen (TAA), β-galactosidase (β-gal) to obtain the lethal subclone CT26.CL25. The growth rate and lethality of CT26.CL25 and CT26.WT is virtually identical despite the expression by CT26.CL25 of the model TAA, β-galactosidase, in normal mice.
CommentsThe cells are assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests are negative.
Culture Conditions and Handling
Culture MediumDulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS), 10%
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: In order to avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersal.
  4. Add 6.0-8.0 mL of complete growth medium and then aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to the new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation RatioThe ratio of 1:4 to 1:10 is recommended.
Fluid RenewalEvery 2 to 3 days.
Freeze MediumComplete growth medium 95%; DMSO, 5%
Culture Temperature37°C
AtmosphereCarbon dioxide (CO2), 5%; air, 95%

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For research use only. Not intended for any clinical use.

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