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CT26 Cell Line

General Information
OrganismMus musculus,mouse
SynonymsCT-26;CT 26;CT-26 WT
Cell Line DescriptionCT26 is a murine colorectal carcinoma cell line which is from a BALB/c mouse.The cell is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line.These cells are adherent and have a fibroblast morphology.They will form tumors and metastases post implantation into syngenic BALB/c mice or immunocompromised mice.
TissueColon
DiseaseMouse colon adenocarcinoma
StrainBALB/c
Cell TypeFibroblast
Product FormatFrozen
Growth ModeAdherent
ApplicationsCT26 cells are suitable forin vitroandin vivoexperimentation.These syngenic BALB/c mice and immunocompromised mice can be used for life studies,and will form tumors following implantation of the cells.
Shipped inDry ice
Storage Temperature−196°C
Culture Conditions and Handling
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25%(w/v)Trypsin-0.03%(w/v)EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.Note:In order to avoid clumping,do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.Cells that are difficult to detach can be placed at 37°C to facilitate dispersal.
  4. Add 6.0-8.0 mL of complete growth medium and then aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to the new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation RatioThe ratio of 1:4 to 1:10 is recommended.
Fluid RenewalEvery 2 to 3 days.
Freeze MediumComplete growth medium 95%;DMSO,5%
Culture Temperature37°C
AtmosphereCarbon dioxide(CO2),5%;air,95%
Cat.No.Product NamePrice
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CSC-RO0017 mSlc5a5 Overexpression Stable Cell Line-CT26 Inquiry
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CSC-RO0632 Human CLDN18-A2 Stable Cell Line - CT26 Inquiry
CSC-RO0596 Human VTCN1 Stable Cell Line - CT26 Inquiry
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CSC-RO0617 Human CD19 Stable Cell Line - CT26 Inquiry
CSC-RO0620 Human SIGLEC15 Stable Cell Line - CT26 Inquiry
CSC-RO0623 Human LILRB2 Stable Cell Line - CT26 Inquiry
CSC-RO0448 Human ERBB2 Stable Cell Line - CT26 Inquiry
CSC-RO0451 Human EGFR Stable Cell Line - CT26 Inquiry
CSC-RO0675 Luc-Human-CXADR Stable Cell Line - CT26 Inquiry
CSC-RO0690 Mouse Fap Stable Cell Line - CT26 Inquiry
CSC-RO0841 Human FGFR2 Stable Cell Line - CT26 Inquiry
CSC-RO0843 Human NT5E/CD274 Stable Cell Line - CT26 Inquiry
CSC-RO0855 Human NT5E Stable Cell Line - CT26 Inquiry
CSC-RO0859 Human GDF15 Stable Cell Line - CT26 Inquiry
CSC-RO0877 Human PVR Stable Cell Line - CT26 Inquiry
CSC-RO0881 Human MELTF Stable Cell Line - CT26 Inquiry
CSC-RO0887 Human ENTPD1 Stable Cell Line - CT26 Inquiry
CSC-RO0904 Human KLRD1/KLRC1 Stable Cell Line - CT26 Inquiry
CSC-RO0936 Mouse SIGLEC15 Stable Cell Line - CT26 Inquiry
CSC-RG1798 Human CCR5 Stable Cell Line - CT26 Inquiry
CSC-RG1808 Human CCR8 Stable Cell Line - CT26 Inquiry
CSC-RG1816 Human GPRC5D Stable Cell Line - CT26 Inquiry
CSC-RO0959 Human ROR1 Stable Cell Line - CT26 Inquiry
CSC-RO0983 Human SLC39A6 Stable Cell Line - CT26 Inquiry
CSC-RO0987 Human FOLH1 Stable Cell Line - CT26 Inquiry
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CSC-RH0114M Humanized CD24 Murine Tumor Cell Line Inquiry
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CSC-RO1150M Cas9 Stable Cell Line - CT26 Inquiry
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CSC-RO01460 OVAL Stable Cell Line - CT26 Inquiry
CSC-RO01713 GFP/OVAL Stable Cell Line - CT26 Inquiry
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* For research use only. Not intended for any clinical use.
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