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General Information | |
Organism | Mus musculus, mouse |
Synonyms | CT-26; CT 26; CT-26 WT |
Cell Line Description | CT26 is a murine colorectal carcinoma cell line which is from a BALB/c mouse. The cell is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. These cells are adherent and have a fibroblast morphology. They will form tumors and metastases post implantation into syngenic BALB/c mice or immunocompromised mice. |
Tissue | Colon |
Disease | Mouse colon adenocarcinoma |
Strain | BALB/c |
Cell Type | Fibroblast |
Product Format | Frozen |
Growth Mode | Adherent |
Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Applications | CT26 cells are suitable for in vitro and in vivo experimentation. These syngenic BALB/c mice and immunocompromised mice can be used for life studies, and will form tumors following implantation of the cells. |
Shipped in | Dry ice |
Storage Temperature | −196°C |
Characteristics | |
Images | ![]() |
Tumorigenic | Yes |
Distributibility | Yes |
Effects | Yes, in BALB/c mice. Mice are inoculated, subcutaneously, developed lethal tumors at 80% frequency with 103 cells and at 100% with 104 cells. Pulmonary metastases develop when mice are inoculated, intravenously, with 104 cells. |
Subclone CT26.CL25 | CT26.WT is stably transduced with the retroviral vector LXSN that contains the lacZ gene encoding the model tumor associated antigen (TAA), β-galactosidase (β-gal) to obtain the lethal subclone CT26.CL25. The growth rate and lethality of CT26.CL25 and CT26.WT is virtually identical despite the expression by CT26.CL25 of the model TAA, β-galactosidase, in normal mice. |
Comments | The cells are assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests are negative. |
Culture Conditions and Handling | |
Culture Medium | Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS), 10% |
Subculturing |
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Subcultivation Ratio | The ratio of 1:4 to 1:10 is recommended. |
Fluid Renewal | Every 2 to 3 days. |
Freeze Medium | Complete growth medium 95%; DMSO, 5% |
Culture Temperature | 37°C |
Atmosphere | Carbon dioxide (CO2), 5%; air, 95% |
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