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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | CAL 27 is an important human epithelial cell line established by J. Gioanni in 1982 at the Antoine-La Casagrande Center in Nice, France. Derived from a lesion in the middle of the tongue of a 56-year-old Caucasian male (before treatment), this cell line has become one of the most commonly used models in oral cancer research. These cells are polygonal in shape and rich in intracytoplasmic granules. Biologically, CAL 27 is characterized by rapid growth and high malignancy. Although traditionally classified as an oral squamous cell carcinoma (OSCC) cell line, some histopathological studies of CAL 27-derived xenografts have shown that its phenotype is more adenosquamous due to the formation of vesicles in vivo. |
| Tissue | Tongue |
| Disease | Squamous Cell Carcinoma; Adenosquamous Carcinoma |
| Morphology | Epithelial; Polygonal |
| Gender | Male |
| Age | 56 years |
| Ethnicity | Caucasian |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 |
| Applications | 1. Oral Carcinogenesis Research 2. Drug Screening and Sensitivity 3. Angiogenesis and Metastasis Studies 4. Cell Adhesion and Signaling 5. Inflammasome and Apoptosis Analysis |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Aneuploid; modal chromosome number = 43; exhibits complex genomic instability. |
| Tumorigenic | Yes, highly tumorigenic in nude mice; solid tumors typically develop within 6 weeks of subcutaneous inoculation. |
| Mutations | Harbors specific genomic alterations common in oral cancer, including mutations in the TP53 tumor suppressor gene. |
| Markers Expressed | Strong positive staining for keratin; expresses epithelial membrane antigen (EMA) and various integrin subunits. |
| Growth Properties | Doubling time is approximately 45 hours; does not grow well in semi-solid media. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly rinse the cell layer with 1× PBS (calcium and magnesium ions-free) to remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T25 culture flasks). 4. Observe under an inverted microscope until the cell layer disperses (usually 2 to 10 minutes). For cells that are difficult to separate, incubate at 37°C to promote detachment. 5. Add an equal volume (or twice the volume) of complete culture medium to neutralize the trypsin. 6. Gently pipette the cells and transfer them to centrifuge tubes. 7. Centrifuge at 300×g for 3-5 minutes. 8. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers. |
| Medium Renewal | Every 2 to 3 days. |
| Subcultivation Ratio | A split ratio of 1:2 to 1:6 is recommended (1:6 is preferred by some repositories to maintain health). |
| Culture Medium | DMEM (Dulbecco's Modified Eagle Medium) + 10% Fetal Bovine Serum (FBS) + 1% Penicillin-Streptomycin. Some protocols use high-glucose DMEM (4.5 g/L). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | 90% complete growth medium supplemented with 5% to 10% (v/v) DMSO or specialized freezing media. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02553 | Human SEMA3G Stable Cell Line - Cal-27 | Inquiry |
| CSC-RR00745 | Luciferase Reporter Cell Line - CAL 27 | Inquiry |
| CSC-RR00746 | GFP Reporter Cell Line - CAL 27 | Inquiry |
| CSC-RR00997 | GFP/Luc Reporter Cell Line - CAL 27 | Inquiry |
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