ATDC5 Cell Line

Brief Introdution
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	The ATDC5 cell line is derived from mouse teratocarcinoma cells and is characterized as a chondrogenic cell line that undergoes a sequential process similar to chondrocyte differentiation. During the culture of ATDC5 cells, cartilage nodules are formed through cell condensation. These cells then exhibit a chondrocyte phenotype, secreting type II collagen, aggrecan, and other ECM molecules (early differentiation). This is followed by the appearance of hypertrophic chondrocytes, associated with increased expression of type X collagen, and subsequent matrix mineralization processes. ATDC5 cells are easy to proliferate, so researchers can obtain large numbers of cells to establish an in vitro culture system to simulate cell condensation during cartilage formation in vivo. Furthermore, these cells can remain undifferentiated during expansion. Therefore, the well-characterized chondrogenic cell line ATDC5 is an excellent model to study the molecular mechanisms of chondrogenesis in vitro.
Tissue	Embryo
Disease	Teratocarcinoma
Morphology	Epithelial-like
Product Format	Frozen
Growth Mode	Adherent
Applications	1. Cartilage and bone development studies 2. Drug testing 3. Disease modeling 4. Analysis of gene function 5. Protein signaling studies 6. Tissue engineering 7. 3D cell culture
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	The ATDC5 cell line has been used as an in vitro model in nearly 300 published studies to date. Atsumi et al. reported that ATDC5 cells, isolated from mouse teratocarcinoma fibroblastic cells, could exhibit chondrogenic differentiation at a high frequency, compared with other established cell lines (C3H10T1/2 and RJC3.1). Subsequently, in vitro studies by Shukunami et al. demonstrated that ATDC5 cells can undergo cell condensation and sequential chondrogenic differentiation when treated with insulin, characterized by proteoglycan synthesis and type II collagen expression.
Culture Conditions and Handling
Subculturing	1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3. Add 2.0-3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until the cell layer is dispersed. 4. Add 6.0-8.0 mL of complete growth medium, and then aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to the new culture vessel. 6. Incubate cultures at 37°C.
Medium Renewal	Every 2-3 days
Culture Medium	DMEM: Ham's F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%;Temperature: 37°C
Cryopreservation	Frozen with 70% medium, 20% FBS, 10% DMSO

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Cat.No.	Product Name	Price
CSC-RO01164	Cas9 Stable Cell Line - ATDC5	Inquiry
CSC-RR00661	Luciferase Reporter Cell Line - ATDC5	Inquiry
CSC-RT2598	Mouse P2ry2 Knockout Cell Line-ATDC5	Inquiry

* For research use only. Not intended for any clinical use.

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