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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HT1080 is a fibrosarcoma cell line that has been widely used in biomedical research. The cell line was created from a fibrosarcoma biopsy from a 35-year-old man. The patients who provided the samples had not received radiation or chemotherapy, making it unlikely that unwanted mutations were introduced into the cell lines. HT-1080 cells have been used extensively in research involving cell migration, invasion assays, and testing of anticancer compounds. HT-1080 cells are also used in studies focusing on the extracellular matrix, angiogenesis, and the role of various genes and proteins in cancer progression. HT-1080 cells produce matrix metalloproteinases (MMPs), enzymes that degrade components of the extracellular matrix and play a key role in tumor invasion and metastasis. This property makes the HT-1080 cell line useful for studying the regulation of MMPs and their inhibitors. |
| Tissue | Connective tissue |
| Disease | Fibrosarcoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 35 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Genetic studies 3. Metastasis studies 4. Drug testing 5. Virus research 6. Protein expression studies |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Misra et al. exploited the ability of the HT1080 cell line to form aggressive angiogenic tumors in xenograft mice to study hypoxia-induced tumor angiogenesis. Data show that HIF-1α mediates the upregulation of neuropilin-1 (NRP-1) during hypoxia, leading to angiogenic mimicry and tumor mimicry. Hanyu et al. used HT1080 xenografts to study imaging of angiogenesis in vivo. The team used an antibody against VEGF to inhibit angiogenesis and found that the antibody also reduced lung metastases using fluorescence in vivo to image tumor progression. HT1080 cells can form pseudovascular structures and determined that the protease ADAMTS1 is critical for cells to display endothelial-like genetic signatures. |
| Characteristics | |
| Cytogenetic Information | Pseudodiploidy is frequently noted. Approximately 40% of the cells have a rearranged karyotype in which an extra group E chromosome and a group C chromosome (probably chromosome 11) are missing. |
| Tumorigenic | Yes, in immunosuppressed mice. |
| Oncogenes | ras+ |
| Virus Susceptibility | poliovirus 1, vesicular stomatitis, RD114, feline leukemia virus (FeLV) |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, resuspend cells by gently mixing with 10 ml of medium and centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | Every 3 days |
| Subcultivation Ratio | The ratio of 1:4 to 1:8 is recommended. |
| Culture Medium | 90% DMEM + 10% h.i. FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
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