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Baculovirus-Insect Cell Expression System

Creative Biogene is a biotechnology company which has the expertise and experience in producing custom proteins using Baculovirus-Insect cell expression system. Creative Biogene's sophisticated equipments, advanced technologies and highly experienced staffs are available to provide you with the complete protein production and purification service, including sequence optimization and synthesis, recombinant bacmid construction, baculovirus generation, flexible scale protein production and purification, post processing of purified protein et al. Creative Biogene is confident in providing you with the most affordable, high-quality gene expression services using Baculovirus-Insect cell expression system in a short turnaround time.

Baculovirus-Insect cell expression system is one of the most popular eukaryotic expression systems for research and industrial applications. There are several advantages of using the baculovirus-Insect cell expression system, such as improved solubility, ability to incorporate post-translational modifications, and higher yield of secreted proteins. Baculovirus-Insect cell expression system is widely used to express recombinant protein kinase and protein complex. It is suitable for customers who prefer a eukaryotic expression system, are exploring lower-cost alternative to mammalian expression system but do not want to compromise on the overall quality of the target protein. With years of development, Creative Biogene can provide customers worldwide gene expression service using Baculovirus-Insect cell expression system by establishing the best possible protocol for your project and then scale up to get the highest expression of your target protein.

Applications:

  • Eukaryotic protein analysis, e.g. structural studies
  • Research tool proteins for crystallography
  • Large-scale protein production for feed studies
  • Production of large and/or complex proteins

Features:

  • One-stop solution from gene synthesis/gene cloning to purified protein.
  • Highly experienced staffs and mature technical platforms can ensure high-level expression of both intracellular and secreted recombinant proteins.
  • Optimum Conditions: various insect cell lines, harvest at different time point and test different MOI to guarantee the highest yield.
  • Short timeline from gene synthesis/cloning to protein recovery.
  • Cost-saving: provide high quality proteins with competitive price.

Creative Biogene gene expression production service based on Baculovirus-Insect cell expression system, which includes:

  • Codon optimization for any given expression system (upon request)
  • Gene synthesis, subclone gene of interest into expression vector
  • Bacmid DNA preparation and infecting insect cell
  • High titer virus seed preparation
  • Pilot protein expression and purification
  • Large scale protein expression and purification

References:

  1. Jarvis DL (2009). Baculovirus–insect cell expression systems. Methods in enzymology, 463, 191-222.
  2. Lahtinen T, et al. (2008). Hydrophobin (HFBI): A potential fusion partner for one-step purification of recombinant proteins from insect cells. Protein expression and purification, 59(1), 18-24.
  3. Ciccarone VC, et al. (1998). Generation of recombinant baculovirus DNA in E. coli using a baculovirus shuttle vector. Molecular diagnosis of infectious diseases, 213-235.

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