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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | WERI-Rb-1 is a human retinoblastoma cell line established in 1974, derived from a primary intraocular tumor in a 1-year-old Caucasian female patient. It is recognized as one of the most widely accepted models for studying the biological characteristics of retinoblastoma. Retinoblastoma is a rare form of cancer that develops rapidly from immature cells within the retina. WERI-Rb-1 cells typically grow in suspension, forming multicellular clusters or grape-like aggregates. This cell line is characterized by the absence of functional RB1 (retinoblastoma) protein—resulting from genetic mutation—which is also a hallmark feature of the disease. It serves as an indispensable tool and is widely utilized in research fields such as neuro-oncology, cell cycle regulation, and the development of targeted therapies for pediatric ocular tumors. |
| Tissue | Eye; Retina |
| Disease | Retinoblastoma |
| Morphology | Lymphoblast-like; grows in clusters |
| Gender | Female |
| Age | 1 year |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Retinoblastoma pathogenesis and genetic research 2. Study of RB1 gene function and cell cycle regulation 3. Drug screening for pediatric ocular malignancies 4. Investigation of photoreceptor differentiation and neural signaling 5. Evaluation of novel intraocular delivery systems for chemotherapy |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid; modal number = 47; range = 45 to 49 |
| Tumorigenic | Yes, in immunocompromised (nude) mice (intraocular or subcutaneous) |
| Genetic Profile | RB1 mutation/deletion (null); TP53 wild-type; MYCN amplification in some sublines |
| Expression Markers | Positive for neuron-specific enolase (NSE) and S-100 protein |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Cell cultures can be maintained by adding fresh medium or by replacing the medium. 2. If the medium requires replacement, centrifuge the cell suspension at 125 × g for 5 to 10 minutes. 3. Resuspend the cell pellet in fresh complete growth medium. 4. To obtain single cells or smaller cell clusters, gently pipette the cell clumps several times. 5. Maintain the cell density between 2 × 10^5 and 1 × 10^6 viable cells/mL. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00858 | Luciferase Reporter Cell Line - WERI-Rb-1 | Inquiry |
| CSC-RR00875 | GFP/Luc Reporter Cell Line - WERI-Rb-1 | Inquiry |
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