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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | RS4-11 is a lymphoblastoid cell line isolated from the bone marrow of a 32-year-old Caucasian female patient with acute lymphoblastic leukemia. It is useful in the study of immunology and immune system disorders. These cells lack surface and cytoplasmic immunoglobulins and are negative for CALLA (CD10). RS4-11 cells are also negative for myeloperoxidase and chloroacetate esterase and do not stain with Sudan black. In addition, these cells are strongly positive for terminal deoxynucleotidyl transferase (TdT) and are positive for BA-1 (CD24), BA-2 (CD9), and MHC class II antigens (HLADR). |
| Tissue | Bone; Marrow |
| Disease | Acute lymphoblastic leukemia (ALL) |
| Morphology | Lymphoblast |
| Gender | Female |
| Age | 32 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Testing and Development 3. Genetic Studies 4. Pathway Analysis 5. Epigenetic Research 6. Immunotherapy Research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The RS4-11 cell line is a suitable in vitro model for studying leukemias carrying t(4;11) because the original cytogenetic abnormality is present and preserved. As with other cell lines, the karyotype evolves during long-term culture through the accumulation of secondary mutations and rearrangements. |
| Characteristics | |
| Karyotype | RS4-11 cells have a characteristic chromosomal abnormality, t(4;11)(q21;q23), and isochromosomes of the long arm of chromosome 7. |
| Antigen Expression | HLA DR +; CD9 +; CD24 + |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Cultures can be maintained by adding fresh medium or changing the medium. Alternatively, cultures can be established by centrifugation and resuspending at 1 X 10^5 viable cells/mL. Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/mL. |
| Medium Renewal | Every 2 to 3 days. |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | RPMI-1640 + 10% FBS + 1% P/S |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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