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Adeno-associated virus (AAV) is a powerful vector system used to transduce cells in vivo. Recombinant AAV can efficiently deliver genetic material to both dividing and non-dividing cells. AAV is replication-defective, nonpathogenic, and noncytotoxic, and can maintain physiologically relevant expression levels in cells and tissues. Currently, AAV has become one of the most promising viral vectors for gene therapy.
Creative Biogene offers a comprehensive range of recombinant AAV reporter particles featuring over 12 serotypes and engineered capsid variants. These AAVs achieve efficient delivery of fluorescent and bioluminescent reporter genes to diverse cell types in vitro and in vivo. Creative Biogene's reporter AAVs include high-titer production using proprietary HEK293 manufacturing platforms, extensive quality control testing, and custom packaging capabilities. Fluorescent reporter options such as GFP, RFP, mCherry, and luciferase reporters provide sensitive readouts of transduction efficiency and gene expression. The AAV reporter particles represent powerful tools for tracking virus biodistribution, optimizing delivery conditions, and studying cell biology and gene function.
Creative Biogene's diverse selection of AAV reporter particles, incorporating fluorescent, luminescent, and chromogenic marker genes driven by constitutive or cell-specific promoters, offers customizable and versatile tools for researchers seeking to understand AAV transduction tropism, biodistribution, expression kinetics, and genomic integration profiles in target cells and tissues, as well as gauge the overall delivery efficiency of AAV-based gene therapy vectors.
You can use our AAV reporting particles to achieve the following work:
Case Study 1
Treatment options for chronic spinal cord injury (SCI) remain limited due to unfavorable changes in the microenvironment. Adeno-associated virus (AAV) vectors are potential candidates for use in chronic SCI, considering their safety and stable gene expression in vivo. The researchers tested AAV serotypes expressing a luciferase reporter in a chronic spinal cord injury mouse model. AAVrh10 gave the highest signal and showed favorable neuronal and glial transduction. It also yielded wider expression and higher fluorescence than other serotypes. Thus, AAVrh10 is promising for gene therapy in chronic spinal cord injury.
Figure 1. The researchers designed an AAV vector expressing a luciferase-fluorescent reporter to analyze cell tropism. It contained AAV2 ITRs, CMV promoter, intron, and polyA signal. (Hoshino Y, et al., 2019).
Case Study 2
To compare the transduction efficiency of individual AAV serotypes, green fluorescent protein (GFP) expression was detected by fluorescence microscopy or western blotting. The researchers compared AAV serotypes for cartilage transduction using a GFP reporter. AAV2 and AAV6.2 gave the highest GFP expression in cartilage cells by microscopy and Western blotting. These serotypes may enable gene therapy to treat osteoarthritis.
Figure 2. The researchers evaluated AAV transduction efficiency across serotypes using GFP expression. (Yoon D S, et al., 2021)
Q: What are Reporter AAV Particles?
A: Reporter AAV particles refer to recombinant adeno-associated viruses (rAAVs) that have been engineered to express reporter genes.
Q: What AAV reporter gene expression systems are currently available?
A: The major categories of AAV reporter gene systems include:
(1) Fluorescent proteins: AAV-GFP, AAV-BFP, AAV-RFP, AAV-YFP.
(2) Bioluminescent proteins: AAV-Luciferase, AAV-Gaussia luciferase, AAV-Renilla luciferase.
(3) Fluorogenic enzymes: AAV-β-galactosidase, AAV-β-lactamase.
(4) Chromogenic enzymes: AAV-LacZ - β-galactosidase, AAV-Alkaline phosphatase
(5) Fluorescent proteins coupled to substrates: AAV-GFP-LC3, AAV-mCherry-Histone H2B.
(6) Epitope tags: AAV-FLAG, AAV-c-Myc, AAV-HA, AAV-V5, AAV-His.
Q: What is AAV-Luciferase and what are its applications?
A: AAV-Luciferase utilizes the luciferase gene from fireflies to catalyze a light-producing reaction inside transduced cells. By using bioluminescent imaging, researchers can track the location and persistence of AAV-Luciferase expression non-invasively over time in cell culture and animal models. This provides spatial and temporal information about AAV transduction that is useful for gene therapy research.
Q: What is AAV-FLAG and what are its applications?
A: AAV-FLAG utilizes the short FLAG epitope tag, which can be fused to a protein of interest. Cells transduced with AAV-FLAG will express the FLAG-tagged protein, which can then be detected using anti-FLAG antibodies. This allows researchers to visualize the expression pattern and subcellular localization of the tagged protein by immunohistochemistry or immunofluorescence. AAV-FLAG is useful for studying the biology of AAV-delivered transgenes.
| Cat.No. | Product Name | Price |
|---|---|---|
| AAV00022Z | GFP Adeno-associated virus(AAV Serotype 1) | Inquiry |
| AAV00023Z | LacZ Adeno-associated virus(AAV Serotype 1) | Inquiry |
| AAV00025Z | GFP Adeno-associated virus(AAV Serotype 2) | Inquiry |
| AAV00026Z | LacZ Adeno-associated virus(AAV Serotype 2) | Inquiry |
| AAV00029Z | GFP Adeno-associated virus(AAV Serotype 5) | Inquiry |
| AAV00030Z | LacZ Adeno-associated virus(AAV Serotype 5) | Inquiry |
| AAV00033Z | GFP Adeno-associated virus(AAV Serotype 6) | Inquiry |
| AAV00034Z | LacZ Adeno-associated virus(AAV Serotype 6) | Inquiry |
| AAV00035Z | LacZ Adeno-associated virus(AAV Serotype 8) | Inquiry |
| AAV00038Z | GFP Adeno-associated virus(AAV Serotype 8) | Inquiry |
| AAV00041Z | GFP Adeno-associated virus(AAV Serotype 9) | Inquiry |
| AAV00042Z | LacZ Adeno-associated virus(AAV Serotype 9) | Inquiry |
| AAV00049Z | CAG-GFP Adeno-associated virus(AAV Serotype 1) | Inquiry |
| AAV00051Z | CAG-GFP Adeno-associated virus(AAV Serotype 2) | Inquiry |
| AAV00053Z | CAG-GFP Adeno-associated virus(AAV Serotype 5) | Inquiry |
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