Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | LNCaP cells are androgen-sensitive human prostate adenocarcinoma cells derived from a left supraclavicular lymph node metastasis in 1977 in a 50-year-old white man. They are adherent epithelial cells that grow in aggregates and single cells. High-affinity specific androgen and estrogen receptors are present in both cytoplasmic and nuclear fractions. The LNCaP line is hormone responsive, as demonstrated by the regulation of cell growth and acid phosphatase production by 5α-dihydrotestosterone in vitro. LNCaP cells also express prostate-specific antigen (PSA). In vivo, male mice develop tumors earlier and more frequently than female mice, and hormonal manipulation has shown that the frequency of tumor development correlates with serum androgen levels. |
| Tissue | Prostate |
| Disease | Carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 50 years |
| Product Format | Frozen |
| Growth Mode | Adherent, single cells and loosely attached clusters |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Study of prostate cancer biology Drug screening Hormone sensitivity studies Development of personalized cancer therapies |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The LNCaP cells grow readily in vitro (up to 8 x 105 cells/sq cm; doubling time, 60 hr), form clones and are highly resistant to human fibroblast interferon. |
| Characteristics | |
| Karyotype | This is a hypotetraploid human cell line. The chromosome mode of LNCaP cells ranged from 76 to 91, indicating a human male karyotype with multiple marker chromosomes. |
| Genes Expressed | Human prostatic acid phosphatase; prostate specific antigen |
| Tumorigenic | Yes, the cells are tumorigenic in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. Maintain the cell concentration of the culture between 1 X 104 and 2 X 105 cells/cm2. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Twice per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Culture Conditions | Air: 95%; Carbon dioxide (CO2): 5%; Temperature: 37℃ |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02621 | Cas9 Stable Cell Line - LNCaP | Inquiry |
| CSC-RO0699 | Human FAP/Luciferase Stable Cell Line - LNCaP | Inquiry |
| CSC-RR00964 | Luciferase Reporter Cell Line - LNCaP | Inquiry |
| CSC-RR0118 | GFP Stable Cell Line-LNCAP | Inquiry |
| CSC-RR0356 | RFP Reporter Cell Line - LNCaP | Inquiry |
| CSC-RT2625 | Human BRCA2 Knockout Cell Line-LNCaP | Inquiry |
| CSC-RT2723 | Human ATF3 Knockout Cell Line-LNCap | Inquiry |
Copyright © Creative Biogene. All rights reserved.