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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | NCI-H82 is an epithelioid cell line isolated from the lung of a 40-year-old Caucasian male with lung cancer. The cell line was isolated in 1978 by A.F. Gazdar and colleagues from the pleural effusion of a patient with small cell lung cancer. The morphology of the original tumor does not conform to the characteristics of small cell lung cancer (SCLC). The cell line is biochemically and morphologically a variant of SCLC, expressing neuron-specific enolase and the brain isoenzyme of creatine kinase. It has no detectable levels of L-DOPA decarboxylase or bombardin. NCI-H82 cells produce an abnormally sized p53 mRNA (3.7 kb). The c-myc DNA sequence is amplified approximately 25-fold and c-myc RNA is increased 24-fold compared to normal cells. NCI-H82 cells are reported to express functional ANP receptors, but treatment with ANP does not alter their growth pattern. |
| Tissue | Lung |
| Disease | Carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 40 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Screening and Development 3. Molecular and Genetic Studies 4. Resistance Mechanisms 5. Biomarker Discovery 6. Tumor Microenvironment Studies 7. Immunotherapy Research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Addtional Info | The NCI-H82 cells stain positively for neurofilaments and vimentin. There is expression of v-fes, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs. |
| Characteristics | |
| Karyotype | This is a near-triploid human cell line. The modal chromosome number is 58, the prevalence is 44%, and the prevalence of polyploidy is 3%. Each cell has two copies of the normal X chromosome. No Y chromosome was detected in the Q-banded preparation. |
| Receptors Expressed | Insulin-like growth factor II receptor (IGF II), atrial natriuretic peptide (ANP) |
| Protein Expression | p53 positive |
| Tumorigenic | Yes, in nude mice |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Maintain the culture by regularly adding or replacing the medium. Start the culture at 2 x 10^5 cells/mL and maintain the cell concentration in the range of 1 x 10^5 to 1 x 10^6 cells/mL for optimal growth. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:5 is recommended. |
| Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
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