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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | KTC-1 is a human papillary thyroid carcinoma (PTC) cell line established in 1991, derived from the pleural effusion of a 68-year-old male patient with recurrent metastatic PTC. As a well-characterized model, it is widely utilized to investigate the progression and underlying molecular mechanisms of thyroid malignancies. KTC-1 cells exhibit an epithelial morphology and are known to harbor the BRAF V600E mutation, which leads to the constitutive activation of the MAP kinase signaling pathway. This cell line is particularly valuable in the following research areas: exploring the biological behavior of advanced PTC, evaluating redifferentiation therapies (specifically NIS induction), and screening for novel kinase inhibitors or radiosensitizers targeting metastatic disease. |
| Tissue | Thyroid; derived from metastatic site (Pleural effusion) |
| Disease | Papillary Thyroid Carcinoma (PTC) |
| Morphology | Epithelial |
| Gender | Male |
| Age | 68 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Papillary thyroid carcinoma (PTC) pathogenesis and metastasis research 2. Study of BRAF V600E-mediated MAPK signaling and its inhibition 3. Investigation of iodine metabolism and sodium iodide symporter (NIS) regulation 4. Drug screening for targeted therapy in radioiodine-refractory thyroid cancer 5. Development of human tumor xenograft models in immunocompromised mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Genetic Profile | BRAF V600E mutation (heterozygous); TP53 wild-type |
| Expression Markers | Positive for thyroid-specific transcription factors (e.g., PAX8) |
| Growth Kinetics | Stable growth rate; forms typical epithelial monolayers |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00787 | Luciferase Reporter Cell Line - KTC-1 | Inquiry |
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