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HCT116 Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionHCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases. In vitro, HCT116 cells grow with a doubling time of about 18 hours. They are suitable for in vivo and in vitro experimentation. Immunocompromised mice can be used for in life studies, and will form tumors and metastases following implantation of the cells.
TissueColon
MorphologyEpithelial
DiseaseColorectal carcinoma
Product FormatFrozen
Growth ModeAdherent
GenderMale
ApplicationsThe cell line has been used to:
  1. study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA);
  2. study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labeled siRNA;
  3. determine the effect of polyamine transport system (PTS) in the selective uptake of Quilamine HQ1-44, an iron chelator;
  4. tumourigenicity studies.
Shipped inDry ice
Storage Temperature−196°C
Culture Conditions and Handling
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to remove all traces of serum, which can inhibit trypsin activity.
  3. Add 2.0-3.0 ml of pre-warmed Trypsin/EDTA solution to the flask, and use a microscope to periodically check the cells until the cell layer is dispersed (about 5-15 minutes). Note: In order to avoid clumping, it is preferable to avoid agitating the cells by bumping or shaking the flask during trypsinization. Difficult to detach cells can be placed in an incubator at 37°C to aid in detachment.
  4. Add 7.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Aliquot the dispersed cells into new culture vessels.
  6. Incubate cultures at 37°C and 5% CO2.
Split RatioA subcultivation ratio of 1:3 to 1:8 is recommended
Medium RenewalEvery 2 to 3 days
Incubation ConditionCarbon dioxide (CO2), 5%; 37°C
Growth ConditionsGrowth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.
Freeze MediumComplete growth media 45%; USDA approved FBS 50%; DMSO 5%
Cat.No.Product NamePrice
CSC-RR0120 GFP Stable Cell Line-HCT-116 Inquiry
CSC-RR0121 RFP Stable Cell Line-HCT-116 Inquiry
CSC-RR0264 Luc/GFP Reporter Cell Line-HCT116 Inquiry
CSC-RR0265 Luc/RFP Reporter Cell Line-HCT116 Inquiry
CSC-RR0267 hSLC5A5/GFP Reporter Cell Line-HCT116 Inquiry
CSC-RR0268 hSLC5A5/Luc Reporter Cell Line-HCT116 Inquiry
CSC-RR0269 hSLC5A5/RFP Reporter Cell Line-HCT116 Inquiry
CSC-RO0021 hSLC5A5 Overexpression Stable Cell Line-HCT116 Inquiry
CSC-RT0034 Human BAX Knockout Cell Line-HCT116 Inquiry
CSC-RT0035 Human CDK2 Knockout Cell Line-HCT116 Inquiry
CSC-RT0036 Human LIG4 (-/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0037 Human NHEJ1 (+/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0038 Human LIG4 (+/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0039 Human AKT2 Knockout Cell Line-HCT116 Inquiry
CSC-RT0040 Human SFN Knockout Cell Line-HCT116 Inquiry
CSC-RT0041 Human TP53 (+/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0043 Human PRKDC (+/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0044 Human BLM Knockout Cell Line-HCT116 Inquiry
CSC-RT0045 Human PTEN Knockout Cell Line-HCT116 Inquiry
CSC-RT0046 Human TP53 (-/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0047 Human KRAS Knockout Cell Line-HCT116 Inquiry
CSC-RT0048 Human APOC1 Knockout Cell Line-HCT116 Inquiry
CSC-RT0049 Human NHEJ1 (-/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0050 Human XRCC4 Knockout Cell Line-HCT116 Inquiry
CSC-RT0051 Human MCTS1 Knockout Cell Line-HCT116 Inquiry
CSC-RT0052 Human PTTG1 Knockout Cell Line-HCT116 Inquiry
CSC-RT0053 Human ZBTB33 Knockout Cell Line-HCT116 Inquiry
CSC-RT0054 Human XIAP Knockout Cell Line-HCT116 Inquiry
CSC-RT0055 Human DICER1 Knockout Cell Line-HCT116 Inquiry
CSC-RT0056 Human BBC3 Knockout Cell Line-HCT116 Inquiry
CSC-RT0057 Human XRCC6 Knockout Cell Line-HCT116 Inquiry
CSC-RT0058 Human PPARD Knockout Cell Line-HCT116 Inquiry
CSC-RT0059 Human PRKDC (-/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0060 Human KDM5C Knockout Cell Line-HCT116 Inquiry
CSC-RT0061 Human AKT1 Knockout Cell Line-HCT116 Inquiry
CSC-RT0062 Human USP7 (+/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0063 Human PDPK1 Knockout Cell Line-HCT116 Inquiry
CSC-RT0064 Human DIABLO Knockout Cell Line-HCT116 Inquiry
CSC-RT0065 Human USP7 (-/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0066 Human KDM5A (+/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0067 Human DNMT3B Knockout Cell Line-HCT116 Inquiry
CSC-RT0068 Human KDM5A (-/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0069 Human SMAD4 Knockout Cell Line-HCT116 Inquiry
CSC-RT0070 Human CTNNB1 (+/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0071 Human FBXW7 Knockout Cell Line-HCT116 Inquiry
CSC-RT0072 Human PTEN Knockout Cell Line-HCT116 Inquiry
CSC-RT0073 Human CHEK1 Knockout Cell Line with TP53 wild type background -HCT116 Inquiry
CSC-RT0074 Human CTNNB1 (-/-) Knockout Cell Line-HCT116 Inquiry
CSC-RT0075 Human CHEK1 Knockout Cell Line with TP53 null background-HCT116 Inquiry
CSC-RT0076 Human CDKN1A/BBC3 Knockout Cell Line-HCT116 Inquiry
CSC-RT0077 Human CDK2/TP53 Knockout Cell Line-HCT116 Inquiry
CSC-RT0078 Human DNMT1 Knockout Cell Line-HCT116 Inquiry
CSC-RT0079 Human PRKDC/XRCC6 Knockout Cell Line-HCT116 Inquiry
CSC-RT0080 Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116 Inquiry
CSC-RT0081 Human XRCC6/LIG4 Knockout Cell Line-HCT116 Inquiry
CSC-RT0082 Human AKT1/AKT2 Knockout Cell Line-HCT116 Inquiry
CSC-RT0102 HNRNPA1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1802 ZFYVE16 Knockout Cell Line-HCT116 Inquiry
CSC-RT1854 ATF3 Knockout Cell Line-HCT116 Inquiry
CSC-RT1855 ADAM17 Knockout Cell Line-HCT116 Inquiry
CSC-RT1856 CSNK2A1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1857 SLC9A3R1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1873 AES Knockout Cell Line-HCT116 Inquiry
CSC-RT1874 SQSTM1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1875 PODXL Knockout Cell Line-HCT116 Inquiry
CSC-RT1876 EZR Knockout Cell Line-HCT116 Inquiry
CSC-RT1877 FSCN1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1878 EPHA2 Knockout Cell Line-HCT116 Inquiry
CSC-RT1879 MELK Knockout Cell Line-HCT116 Inquiry
CSC-RT1880 RAF1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1881 PPIB Knockout Cell Line-HCT116 Inquiry
CSC-RT1884 MLST8 Knockout Cell Line-HCT116 Inquiry
CSC-RT1885 RRM2B Knockout Cell Line-HCT116 Inquiry
CSC-RT1886 CDKN1A Knockout Cell Line-HCT116 Inquiry
CSC-RT1889 NFKB2 Knockout Cell Line-HCT116 Inquiry
CSC-RT1897 TSPO Knockout Cell Line-HCT116 Inquiry
CSC-RT1899 NR2F2 Knockout Cell Line-HCT116 Inquiry
CSC-RT1900 ATAD2 Knockout Cell Line-HCT116 Inquiry
CSC-RT1903 PKN2 Knockout Cell Line-HCT116 Inquiry
CSC-RT1912 SMARCC1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1925 GNB1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1929 IL6ST Knockout Cell Line-HCT116 Inquiry
CSC-RT1930 CD2BP2 Knockout Cell Line-HCT116 Inquiry
CSC-RT1931 TMSB10 Knockout Cell Line-HCT116 Inquiry
CSC-RT1939 PLK2 Knockout Cell Line-HCT116 Inquiry
CSC-RT1951 EIF1 Knockout Cell Line-HCT116 Inquiry
CSC-RT1955 UBE2K Knockout Cell Line-HCT116 Inquiry
CSC-RT1968 TUBB3 Knockout Cell Line-HCT116 Inquiry
CSC-RT1969 POR Knockout Cell Line-HCT116 Inquiry
CSC-RT1975 GNAI3 Knockout Cell Line-HCT116 Inquiry
CSC-RT2401 Human GNA13 Knockout Cell Line-HCT116 Inquiry
CSC-RT2515 Human RPS6KB1 Knockout Cell Line-HCT116 Inquiry
CSC-RT2523 Human SET Knockout Cell Line-HCT116 Inquiry
CSC-RT2558 Human UBE2H Knockout Cell Line-HCT116 Inquiry
CSC-RT2564 Human USP21 Knockout Cell Line-HCT116 Inquiry
CSC-RO0222 Cas9 Stable Cell Line - HCT116 Inquiry
CLKO-1651 ZFYVE16 KO Cell Lysate-HCT116 Inquiry
CLKO-1704 ATF3 KO Cell Lysate-HCT116 Inquiry
CLKO-1705 ADAM17 KO Cell Lysate-HCT116 Inquiry
CLKO-1706 CSNK2A1 KO Cell Lysate-HCT116 Inquiry
CLKO-1707 SLC9A3R1 KO Cell Lysate-HCT116 Inquiry
CLKO-1727 AES KO Cell Lysate-HCT116 Inquiry
CLKO-1728 SQSTM1 KO Cell Lysate-HCT116 Inquiry
CLKO-1729 PODXL KO Cell Lysate-HCT116 Inquiry
CLKO-1730 EZR KO Cell Lysate-HCT116 Inquiry
CLKO-1731 FSCN1 KO Cell Lysate-HCT116 Inquiry
CLKO-1732 EPHA2 KO Cell Lysate-HCT116 Inquiry
CLKO-1733 MELK KO Cell Lysate-HCT116 Inquiry
CLKO-1734 RAF1 KO Cell Lysate-HCT116 Inquiry
CLKO-1735 PPIB KO Cell Lysate-HCT116 Inquiry
CLKO-1739 MLST8 KO Cell Lysate-HCT116 Inquiry
CLKO-1740 RRM2B KO Cell Lysate-HCT116 Inquiry
CLKO-1741 CDKN1A KO Cell Lysate-HCT116 Inquiry
CLKO-1744 NFKB2 KO Cell Lysate-HCT116 Inquiry
CLKO-1752 TSPO KO Cell Lysate-HCT116 Inquiry
CLKO-1754 NR2F2 KO Cell Lysate-HCT116 Inquiry
CLKO-1755 ATAD2 KO Cell Lysate-HCT116 Inquiry
CLKO-1758 PKN2 KO Cell Lysate-HCT116 Inquiry
CLKO-1767 SMARCC1 KO Cell Lysate-HCT116 Inquiry
CLKO-1781 GNB1 KO Cell Lysate-HCT116 Inquiry
CLKO-1785 IL6ST KO Cell Lysate-HCT116 Inquiry
CLKO-1786 CD2BP2 KO Cell Lysate-HCT116 Inquiry
CLKO-1787 TMSB10 KO Cell Lysate-HCT116 Inquiry
CLKO-1795 PLK2 KO Cell Lysate-HCT116 Inquiry
CLKO-1807 EIF1 KO Cell Lysate-HCT116 Inquiry
CLKO-1813 UBE2K KO Cell Lysate-HCT116 Inquiry
CLKO-1828 TUBB3 KO Cell Lysate-HCT116 Inquiry
CLKO-1829 POR KO Cell Lysate-HCT116 Inquiry
CLKO-1835 GNAI3 KO Cell Lysate-HCT116 Inquiry
CSC-RR0436 GFP/RFP Reporter Cell Line-HCT116 Inquiry
CSC-RT2653 Human OLA1 Knockout Cell Line-HCT116 Inquiry
CSC-RT2701 Human MTAP Knockout Cell Line-HCT116 Inquiry
CSC-RT2742 Human CDK4 Knockout Cell Line-HCT116 Inquiry
CSC-RO0995 Human CLDN18-A2 Stable Cell Line - HCT116 Inquiry
CSC-RG1830 Human CXCR1 Stable Cell Line-HCT116 Inquiry
CSC-RG1832 Human CXCR2 Stable Cell Line-HCT116 Inquiry
CSC-RT2767 Human BRAF Knockout Cell Line-HCT116 Inquiry
CSC-RT2776 Human WRN Knockout Cell Line-HCT116 Inquiry
CSC-RR0603 Myc-Luc Reporter Cell Line - HCT116 Inquiry
CSC-RR0266-F FLuc Reporter Cell Line - HCT116 Inquiry
CSC-RR0266-G GLuc Reporter Cell Line - HCT116 Inquiry
CSC-RG01847 Human SSTR2 Stable Cell Line - HCT 116 Inquiry
CSC-RO01323 Human IMPDH2 Stable Cell Line - HCT 116 Inquiry
CSC-RO01324 Human SQSTM1 Stable Cell Line - HCT 116 Inquiry
CSC-RO01326 Human CCDC25 Stable Cell Line - HCT 116 Inquiry
CSC-RO01333 Human PPARA Stable Cell Line - HCT 116 Inquiry
CSC-RO01335 Human PADI2 Stable Cell Line - HCT 116 Inquiry
CSC-RO01336 Human DHX9 Stable Cell Line - HCT 116 Inquiry
CSC-RT2807 Human DCAF1 Knockout Cell Line-HCT116 Inquiry
CSC-RO01475 OVAL Stable Cell Line - HCT116 Inquiry
CSC-RO01728 GFP/OVAL Stable Cell Line - HCT116 Inquiry
CSC-RO01981 Luc/OVAL Stable Cell Line - HCT116 Inquiry
CSC-RO02234 GFP/Luc/OVAL Stable Cell Line - HCT116 Inquiry
CSC-RT2813 Human ATM Knockout Cell Line-HCT116 Inquiry
CSC-RO02548 Human HLF Stable Cell Line - HCT116 Inquiry
CSC-RT2818 Human CRBN Knockout Cell Line-HCT116 Inquiry
CSC-RT2825 Human MAPT Knockout Cell Line-HCT116 Inquiry
CSC-RT2827 Human FANCM Knockout Cell Line-HCT116 Inquiry
CSC-RR01102 AP1-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RR01103 ARE-Luc/EF1α-mCherry Reporter Cell Line - HCT116 Inquiry
CSC-RR01104 ISRE-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RR01105 Myc-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RR01106 NFκB-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RR01107 p53-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RR01108 STAT3-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RR01109 IL6pro-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RR01110 TNFαPro-Luc/EF1α-mCherry Reporter Cell Line - HCT 116 Inquiry
CSC-RT2831 Human HIF1A Knockout Cell Line-HCT116 Inquiry
CSC-RT2832 Human FANCI Knockout Cell Line-HCT116 Inquiry
CSC-RT2901 Human TNFSF15 Knockout Cell Line-HCT116 Inquiry

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* For research use only. Not intended for any clinical use.
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