HCT 116 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	HCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases. In vitro, HCT116 cells grow with a doubling time of about 18 hours. They are suitable for in vivo and in vitro experimentation. Immunocompromised mice can be used for in life studies, and will form tumors and metastases following implantation of the cells.
Tissue	Colon
Morphology	Epithelial
Disease	Colorectal carcinoma
Product Format	Frozen
Growth Mode	Adherent
Gender	Male
Applications	The cell line has been used to: study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA); study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labeled siRNA; determine the effect of polyamine transport system (PTS) in the selective uptake of Quilamine HQ1-44, an iron chelator; tumourigenicity studies.
Shipped in	Dry ice
Storage Temperature	−196°C
Culture Conditions and Handling
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to remove all traces of serum, which can inhibit trypsin activity. Add 2.0-3.0 ml of pre-warmed Trypsin/EDTA solution to the flask, and use a microscope to periodically check the cells until the cell layer is dispersed (about 5-15 minutes). Note: In order to avoid clumping, it is preferable to avoid agitating the cells by bumping or shaking the flask during trypsinization. Difficult to detach cells can be placed in an incubator at 37°C to aid in detachment. Add 7.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting. Aliquot the dispersed cells into new culture vessels. Incubate cultures at 37°C and 5% CO₂.
Split Ratio	A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal	Every 2 to 3 days
Incubation Condition	Carbon dioxide (CO₂), 5%; 37°C
Growth Conditions	Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.
Freeze Medium	Complete growth media 45%; USDA approved FBS 50%; DMSO 5%

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Cat.No.	Product Name	Price
CLKO-1651	ZFYVE16 KO Cell Lysate-HCT116	Inquiry
CLKO-1704	ATF3 KO Cell Lysate-HCT116	Inquiry
CLKO-1705	ADAM17 KO Cell Lysate-HCT116	Inquiry
CLKO-1706	CSNK2A1 KO Cell Lysate-HCT116	Inquiry
CLKO-1707	SLC9A3R1 KO Cell Lysate-HCT116	Inquiry
CLKO-1727	AES KO Cell Lysate-HCT116	Inquiry
CLKO-1728	SQSTM1 KO Cell Lysate-HCT116	Inquiry
CLKO-1729	PODXL KO Cell Lysate-HCT116	Inquiry
CLKO-1730	EZR KO Cell Lysate-HCT116	Inquiry
CLKO-1731	FSCN1 KO Cell Lysate-HCT116	Inquiry
CLKO-1732	EPHA2 KO Cell Lysate-HCT116	Inquiry
CLKO-1733	MELK KO Cell Lysate-HCT116	Inquiry
CLKO-1734	RAF1 KO Cell Lysate-HCT116	Inquiry
CLKO-1735	PPIB KO Cell Lysate-HCT116	Inquiry
CLKO-1739	MLST8 KO Cell Lysate-HCT116	Inquiry

* For research use only. Not intended for any clinical use.

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