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HCT 116 Cell Line


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HCT 116 Cell Line

General Information
OrganismHomo sapiens, human
Cell Line DescriptionHCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases. In vitro, HCT116 cells grow with a doubling time of about 18 hours. They are suitable for in vivo and in vitro experimentation. Immunocompromised mice can be used for in life studies, and will form tumors and metastases following implantation of the cells.
TissueColon
MorphologyEpithelial
DiseaseColorectal carcinoma
Product FormatFrozen
Growth ModeAdherent
GenderMale
Biosafety Level1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ApplicationsThe cell line has been used to:
  1. study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA);
  2. study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labeled siRNA;
  3. determine the effect of polyamine transport system (PTS) in the selective uptake of Quilamine HQ1-44, an iron chelator;
  4. tumourigenicity studies.
Shipped inDry ice
Storage Temperature−196°C
Characteristics
KaryotypeNear diploid with the modal number at 45 (62%) and polyploids occurring at 6.8%.
ImagesHCT 116 Cell Line
ProductsNot specified
ReceptorsNot specified
Genes ExpressedCarcinoembryonic antigen (CEA) 1 ng per 106 cells per 10 days.
TumorigenicYes
EffectsYes, in nude mice
CommentsThe cells are positive for keratin by immunoperoxidase staining.
HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression.
Culture Conditions and Handling
Culture MediumDMEM + 2 mM Glutamine + 10% Calf Serum (CS).
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with sterile, 1x PBS, or 0.25% (w/v) Trypsin-0.53% (w/v) EDTA solution in order to remove all traces of serum, which can inhibit trypsin activity.
  3. Add 2.0-3.0 ml of pre-warmed Trypsin/EDTA solution to the flask, and use a microscope to periodically check the cells until the cell layer is dispersed (about 5-15 minutes). Note: In order to avoid clumping, it is preferable to avoid agitating the cells by bumping or shaking the flask during trypsinization. Difficult to detach cells can be placed in an incubator at 37°C to aid in detachment.
  4. Add 7.0-8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Aliquot the dispersed cells into new culture vessels.
  6. Incubate cultures at 37°C and 5% CO2.
Split RatioA subcultivation ratio of 1:3 to 1:8 is recommended
Medium RenewalEvery 2 to 3 days
Incubation ConditionCarbon dioxide (CO2), 5%; 37°C
Growth ConditionsGrowth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.
Freeze MediumComplete growth media 45%; USDA approved FBS 50%; DMSO 5%
Cat.No. Product Name Price
CSC-RR0264Luc/GFP Reporter Cell Line-HCT116Inquriy
CSC-RR0265Luc/RFP Reporter Cell Line-HCT116Inquriy
CSC-RR0266Luc Reporter Cell Line-HCT116Inquriy
CSC-RR0267hSLC5A5/GFP Reporter Cell Line-HCT116Inquriy
CSC-RR0268hSLC5A5/Luc Reporter Cell Line-HCT116Inquriy
CSC-RR0269hSLC5A5/RFP Reporter Cell Line-HCT116Inquriy
CSC-RO0021hSLC5A5 Overexpression Stable Cell Line-HCT116Inquriy
CSC-RT0034Human BAX Knockout Cell Line-HCT116Inquriy
CSC-RT0035Human CDK2 Knockout Cell Line-HCT116Inquriy
CSC-RT0036Human LIG4 (-/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0037Human NHEJ1 (+/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0038Human LIG4 (+/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0039Human AKT2 Knockout Cell Line-HCT116Inquriy
CSC-RT0040Human SFN Knockout Cell Line-HCT116Inquriy
CSC-RT0041Human TP53 (+/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0042Human CDKN1A Knockout Cell Line-HCT116Inquriy
CSC-RT0043Human PRKDC (+/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0044Human BLM Knockout Cell Line-HCT116Inquriy
CSC-RT0045Human PTEN Knockout Cell Line-HCT116Inquriy
CSC-RT0046Human TP53 (-/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0047Human KRAS Knockout Cell Line-HCT116Inquriy
CSC-RT0048Human APOC1 Knockout Cell Line-HCT116Inquriy
CSC-RT0049Human NHEJ1 (-/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0050Human XRCC4 Knockout Cell Line-HCT116Inquriy
CSC-RT0051Human MCTS1 Knockout Cell Line-HCT116Inquriy
CSC-RT0052Human PTTG1 Knockout Cell Line-HCT116Inquriy
CSC-RT0053Human ZBTB33 Knockout Cell Line-HCT116Inquriy
CSC-RT0054Human XIAP Knockout Cell Line-HCT116Inquriy
CSC-RT0055Human DICER1 Knockout Cell Line-HCT116Inquriy
CSC-RT0056Human BBC3 Knockout Cell Line-HCT116Inquriy
CSC-RT0057Human XRCC6 Knockout Cell Line-HCT116Inquriy
CSC-RT0058Human PPARD Knockout Cell Line-HCT116Inquriy
CSC-RT0059Human PRKDC (-/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0060Human KDM5C Knockout Cell Line-HCT116Inquriy
CSC-RT0061Human AKT1 Knockout Cell Line-HCT116Inquriy
CSC-RT0062Human USP7 (+/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0063Human PDPK1 Knockout Cell Line-HCT116Inquriy
CSC-RT0064Human DIABLO Knockout Cell Line-HCT116Inquriy
CSC-RT0065Human USP7 (-/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0066Human KDM5A (+/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0067Human DNMT3B Knockout Cell Line-HCT116Inquriy
CSC-RT0068Human KDM5A (-/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0069Human SMAD4 Knockout Cell Line-HCT116Inquriy
CSC-RT0070Human CTNNB1 (+/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0071Human FBXW7 Knockout Cell Line-HCT116Inquriy
CSC-RT0072Human PTEN Knockout Cell Line-HCT116Inquriy
CSC-RT0073Human CHEK1 Knockout Cell Line with TP53 wild type background -HCT116Inquriy
CSC-RT0074Human CTNNB1 (-/-) Knockout Cell Line-HCT116Inquriy
CSC-RT0075Human CHEK1 Knockout Cell Line with TP53 null background-HCT116Inquriy
CSC-RT0076Human CDKN1A/BBC3 Knockout Cell Line-HCT116Inquriy
CSC-RT0077Human CDK2/TP53 Knockout Cell Line-HCT116Inquriy
CSC-RT0078Human DNMT1 Knockout Cell Line-HCT116Inquriy
CSC-RT0079Human PRKDC/XRCC6 Knockout Cell Line-HCT116Inquriy
CSC-RT0080Human CHEK2 Knockout Cell Line with TP53 wild type background -HCT116Inquriy
CSC-RT0081Human XRCC6/LIG4 Knockout Cell Line-HCT116Inquriy
CSC-RT0082Human AKT1/AKT2 Knockout Cell Line-HCT116Inquriy
CSC-RT1802ZFYVE16 Knockout Cell Line-HCT116Inquriy
CSC-RT1854ATF3 Knockout Cell Line-HCT116Inquriy
CSC-RT1855ADAM17 Knockout Cell Line-HCT116Inquriy
CSC-RT1856CSNK2A1 Knockout Cell Line-HCT116Inquriy
CSC-RT1857SLC9A3R1 Knockout Cell Line-HCT116Inquriy
CSC-RT1873AES Knockout Cell Line-HCT116Inquriy
CSC-RT1874SQSTM1 Knockout Cell Line-HCT116Inquriy
CSC-RT1875PODXL Knockout Cell Line-HCT116Inquriy
CSC-RT1876EZR Knockout Cell Line-HCT116Inquriy
CSC-RT1877FSCN1 Knockout Cell Line-HCT116Inquriy
CSC-RT1878EPHA2 Knockout Cell Line-HCT116Inquriy
CSC-RT1879MELK Knockout Cell Line-HCT116Inquriy
CSC-RT1880RAF1 Knockout Cell Line-HCT116Inquriy
CSC-RT1881PPIB Knockout Cell Line-HCT116Inquriy
CSC-RT1884MLST8 Knockout Cell Line-HCT116Inquriy
CSC-RT1885RRM2B Knockout Cell Line-HCT116Inquriy
CSC-RT1886CDKN1A Knockout Cell Line-HCT116Inquriy
CSC-RT1889NFKB2 Knockout Cell Line-HCT116Inquriy
CSC-RT1897TSPO Knockout Cell Line-HCT116Inquriy
CSC-RT1899NR2F2 Knockout Cell Line-HCT116Inquriy
CSC-RT1900ATAD2 Knockout Cell Line-HCT116Inquriy
CSC-RT1903PKN2 Knockout Cell Line-HCT116Inquriy
CSC-RT1912SMARCC1 Knockout Cell Line-HCT116Inquriy
CSC-RT1925GNB1 Knockout Cell Line-HCT116Inquriy
CSC-RT1929IL6ST Knockout Cell Line-HCT116Inquriy
CSC-RT1930CD2BP2 Knockout Cell Line-HCT116Inquriy
CSC-RT1931TMSB10 Knockout Cell Line-HCT116Inquriy
CSC-RT1939PLK2 Knockout Cell Line-HCT116Inquriy
CSC-RT1951EIF1 Knockout Cell Line-HCT116Inquriy
CSC-RT1955UBE2K Knockout Cell Line-HCT116Inquriy
CSC-RT1968TUBB3 Knockout Cell Line-HCT116Inquriy
CSC-RT1969POR Knockout Cell Line-HCT116Inquriy
CSC-RT1975GNAI3 Knockout Cell Line-HCT116Inquriy
CSC-RT2401Human GNA13 Knockout Cell Line-HCT116Inquriy
CSC-RT2515Human RPS6KB1 Knockout Cell Line-HCT116Inquriy
CSC-RT2523Human SET Knockout Cell Line-HCT116Inquriy
CSC-RT2558Human UBE2H Knockout Cell Line-HCT116Inquriy
CSC-RT0102HNRNPA1 Knockout Cell Line-HCT116Inquriy
CSC-RO0222Cas9 Stable Cell Line - HCT116Inquriy
CLKO-1704ATF3 KO Cell Lysate-HCT116Inquriy
CLKO-1705ADAM17 KO Cell Lysate-HCT116Inquriy
CLKO-1706CSNK2A1 KO Cell Lysate-HCT116Inquriy
CLKO-1707SLC9A3R1 KO Cell Lysate-HCT116Inquriy
CLKO-1727AES KO Cell Lysate-HCT116Inquriy
CLKO-1728SQSTM1 KO Cell Lysate-HCT116Inquriy
CLKO-1729PODXL KO Cell Lysate-HCT116Inquriy
CLKO-1730EZR KO Cell Lysate-HCT116Inquriy
CLKO-1731FSCN1 KO Cell Lysate-HCT116Inquriy
CLKO-1732EPHA2 KO Cell Lysate-HCT116Inquriy
CLKO-1733MELK KO Cell Lysate-HCT116Inquriy
CLKO-1734RAF1 KO Cell Lysate-HCT116Inquriy
CLKO-1735PPIB KO Cell Lysate-HCT116Inquriy
CLKO-1739MLST8 KO Cell Lysate-HCT116Inquriy
CLKO-1740RRM2B KO Cell Lysate-HCT116Inquriy
CLKO-1741CDKN1A KO Cell Lysate-HCT116Inquriy
CLKO-1744NFKB2 KO Cell Lysate-HCT116Inquriy
CLKO-1752TSPO KO Cell Lysate-HCT116Inquriy
CLKO-1754NR2F2 KO Cell Lysate-HCT116Inquriy
CLKO-1755ATAD2 KO Cell Lysate-HCT116Inquriy
CLKO-1758PKN2 KO Cell Lysate-HCT116Inquriy
CLKO-1767SMARCC1 KO Cell Lysate-HCT116Inquriy
CLKO-1781GNB1 KO Cell Lysate-HCT116Inquriy
CLKO-1785IL6ST KO Cell Lysate-HCT116Inquriy
CLKO-1786CD2BP2 KO Cell Lysate-HCT116Inquriy
CLKO-1787TMSB10 KO Cell Lysate-HCT116Inquriy
CLKO-1795PLK2 KO Cell Lysate-HCT116Inquriy
CLKO-1807EIF1 KO Cell Lysate-HCT116Inquriy
CLKO-1813UBE2K KO Cell Lysate-HCT116Inquriy
CLKO-1828TUBB3 KO Cell Lysate-HCT116Inquriy
CLKO-1829POR KO Cell Lysate-HCT116Inquriy
CLKO-1835GNAI3 KO Cell Lysate-HCT116Inquriy
CSC-RT2653Human OLA1 Knockout Cell Line-HCT116Inquriy
CSC-RT2564Human USP21 Knockout Cell Line-HCT116Inquriy
CLKO-1651ZFYVE16 KO Cell Lysate-HCT116Inquriy
CSC-RR0436GFP/RFP Reporter Cell Line-HCT116Inquriy

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