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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | BEAS-2B is an immortalized human bronchial epithelial cell line established by transforming normal human bronchial epithelial cells using an Adenovirus 12-SV40 hybrid virus (Ad12-SV40). Unlike many lung cancer cell lines, BEAS-2B is derived from non-tumorigenic tissue and retains many characteristics of normal lung epithelial cells, including the capacity to undergo squamous differentiation in response to specific stimuli. It is widely recognized as a "near-normal" model within the field of pulmonary research. This cell line serves as an invaluable tool for investigating respiratory toxicology, airway inflammation, the mechanisms of lung carcinogenesis, and cellular responses to environmental pollutants and cigarette smoke. |
| Tissue | Lung; Bronchus |
| Cell Type | Epithelial (Immortalized) |
| Morphology | Epithelial |
| Gender | Not specified |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 (Cells contain polyomaviral DNA sequences) |
| Applications | 1. Respiratory toxicology and environmental health studies 2. Research on early stages of lung carcinogenesis and gene transformation 3. Study of airway inflammation and bronchial epithelial barrier function 4. Screening for protective agents against pulmonary oxidative stress 5. Investigation of viral and bacterial interactions with the human airway |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | No, does not form tumors in immunocompromised mice |
| Immortalization | Transfected with Ad12-SV40 hybrid virus |
| Phenotype | Maintains bronchial epithelial markers; capable of squamous differentiation |
| Usage Note | Highly sensitive to TGF-β, which can induce squamous metaplasia. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically requiring 3 to 7 minutes). 4. Add complete growth medium to neutralize the trypsin, and gently pipette to create a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:8 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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