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Creative Biogene, as one of the leading biotechnology companies, possesses extensive expertise and experience which is able to provide random mutagenesis library construction services to customers worldwide. Our top priorities are to streamline the platform processes or work with you to design a process that meets your specific needs. Creative Biogene's goal is to provide you with the most affordable, high-quality random mutagenesis library construction services to ensure your satisfaction in a timely and professional manner.
Random mutagenesis of DNA is a powerful tool to study gene function and protein structure and is a prerequisite for preparation of randomized DNA libraries, required for many genetic selections. Compared with the rational design, random mutagenesis is not targeted amino acid substitutions, and therefore do not require a large body of the knowledge about the biocatalyst being improved, including the three-dimensional structure and the chemical mechanism of the reaction. Random mutagenesis, introducing random mutations into the protein-coding gene and then screening the expressed mutant proteins, provides access to proteins having altered properties without human bias. Several methods have been developed to create random mutagenesis. Among these, error-prone PCR is one of the most widely used random mutagenesis. During DNA amplification, misincorporation of nucleotides can be promoted by altering the nucleotide ratio and the concentration of divalent cations in the reaction. It has been reported that, by adjusting template concentration, frequency of mutation could be controlled and a library with desired mutation rate could be obtained.
Creative Biogene, as one of the leading biotechnology companies, has years of professional and abundant experience which can construct random mutagenesis libraries by error-prone PCR to satisfy your specific needs. Creative Biogene is a reliable partner that can work with you to accelerate the speed to achieve your research goals. If you have any special requirements in our random mutagenesis library construction services, please feel free to contact us. We are looking forward to working together with your attractive projects.
Applications of random mutagenesis library
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