Pages
Products
Support
CBpromise

Our promise to you:
Guaranteed product quality, expert customer support.

24x7 CUSTOMER SERVICE
CONTACT US TO ORDER

Protocols

protocols
Protocol

CRISPR Plasmid Transfection Protocol

Experiment Tags: CRISPR, CRISPR Plasmid

Experiment Summary: The CRISPR/Cas9 system has two critical parts: sgRNA sequence and Cas9 sequence. These two sequences could be cloned into separated plasmids or combined into one plasmid, each format has its own set of advantages and shares a similar delivery procedure.

protocols
Protocol

T7E1 Assay Protocol

Experiment Tags: T7E1, T7E1 Assay

Experiment Summary: Direct PCR product could be used for T7E1 detection.

protocols
Protocol

Designing sgRNA using CRISPR Protocol

Experiment Tags: sgRNA, CRISPR

Experiment Summary: Selection of sgRNAs for an experiment needs to balance two aspects: maximizing on-target activity while minimizing off-target activity, which sounds obviously but can often require thorough decisions.

protocols
Protocol

Codon Optimization Protocol

Experiment Tags: Codon Optimization, Codon

Experiment Summary: Codon optimization is one of the key steps in achieving the high level expression of target gene. There are some key factors for consideration including transcription efficiency, translation efficiency, gene synthesis and protein folding.

protocols
Protocol

RNAi Protocol

Experiment Tags: RNAi

Experiment Summary: RNAi consists of inititation and effector steps. At inititation steps, the dsRNAs get processed into 21-23 nucleotide (nt) small interfering RNAs (siRNAs) by an RNase III-like enzyme called Dicer.