Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MCF10A is an epithelial cell line isolated in 1984 from the breast of a 36-year-old white woman with fibrocystic breasts. The MCF10A human mammary epithelial cell line is arguably the most commonly used normal breast cell model. These cells originate from benign proliferative breast tissue and become spontaneously immortalized in the absence of defined factors. They are not tumorigenic and do not express estrogen receptors. Their known molecular features include deletions of chromosomal loci containing the p16 and p14ARF genes, which are critical for regulating aging and amplification of the Myc gene. The 3D MCF10A model provides a useful tool for dissecting cell-cell interactions in mammary gland development, studying the impact of the microenvironment on mammary gland cell function, and the impact of different genetic or non-genetic modifications on mammary gland cell transformation. |
| Tissue | Breast |
| Disease | Fibrocystic Disease |
| Morphology | Epithelial |
| Gender | Female |
| Age | 36 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Study of mammary gland development Breast cancer research Drug screening Cell signaling studies Tissue engineering |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | MCF10A cells were positive for epithelial salivary mucin, cytokeratin, and milk fat globule antigen. So far, these cells have shown no signs of terminal differentiation or senescence. MCF10A cell is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF). Observed by electron microscopy, the cells showed characteristics of luminal duct cells but not of myoepithelial cells. MCF10A cells also express breast-specific antigens, detected by positive reactions with MFA-Breast and MC-5 monoclonal antibodies. |
| Characteristics | |
| Isoenzymes | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1-2 PGM3, 1 |
| Tumorigenic | No |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove the medium and rinse the monolayer with calcium- and magnesium-free PBS. 2. Add 3.0 mL of 0.05% trypsin, 0.53 mM EDTA, and incubate at 37℃ for 15 min. 3. To neutralize trypsin, add 3 mL of 0.1% soy trypsin inhibitor solution. 4. Centrifuge the cell suspension at 125 x g for 5 to 10 minutes. 5. Resuspend the cell pellet in complete medium. 6. Add appropriate aliquots of cell suspension to new culture vessels. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:3 to 1:4 is recommended. |
| Culture Medium | Base medium MEBM + 100 ng/ml cholera toxin. |
| Culture Conditions | Air: 95%; Carbon dioxide (CO2): 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 7.5% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RT0032 | Human PTEN Knockout Cell Line-MCF10A | Inquiry |
| CSC-RT0033 | Human TP53 Knockout Cell Line-MCF10A | Inquiry |
Copyright © Creative Biogene. All rights reserved.