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| General Information | |
| Organism | Cricetulus griseus, Chinese hamster |
| Cell Line Description | CHO-S is a suspension culture-adapted subclone derived from the original Chinese hamster ovary (CHO) cell line established by Theodore T. Puck in 1957. Unlike the parental CHO cell line, which grows adherently, the CHO-S variant has been specifically screened and engineered to achieve robust growth in high-density suspension culture in serum-free, chemically defined media. Due to its superior growth kinetics, ease of scale-up in large bioreactors, and ability to perform human-like post-translational modifications, CHO-S has become a cornerstone of the biopharmaceutical industry. This cell line is characterized by high productivity and has received approval from multiple regulatory agencies for therapeutic protein expression. |
| Tissue | Ovary |
| Disease | Normal; Spontaneously immortalized |
| Morphology | Spherical (in suspension); Epithelial-like (when adherent) |
| Gender | Female |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Biopharmaceutical Production 2. High-Yield Transient Expression 3. Vaccine Development 4. Functional Genomics and Engineering 5. Bioprocess Optimization |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Hypodiploid/Aneuploid; modal chromosome number is typically 2n = 20 or 21 (normal hamster 2n = 22); exhibits significant genomic instability and rearrangements over prolonged culture. |
| DNA Profile (STR) | Specific to Cricetulus griseus; verified through species-specific PCR and genomic markers. |
| Genomic Features | Contains "safe harbor" loci such as the H11 locus on chromosome 1q13, which allows for stable and high-level transgene integration without gene silencing. |
| Protein Expression | Capable of complex N-linked and O-linked glycosylation, producing sialic acids similar to human patterns, though lacking certain human-specific enzymes. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Suspension cells do not require enzymatic digestion. 2. Determine viable cell density and viability using a hemocytometer or automated cell counter. 3. Calculate the cell volume required for seeding at the recommended density. 4. Aseptically transfer the cell suspension to a new, sterile, vented Erlenmeyer flask. 5. Add preheated, serum-free culture medium. 6. Place the Erlenmeyer flask on the shaker platform in an incubator. |
| Medium Renewal | Every 2 to 3 days. |
| Subcultivation Ratio | Seed at a density of 0.2 × 10^6 to 0.5 × 10^6 live cells/mL; passage culture was performed when the density reached 1 × 10^6 to 3 × 10^6 cells/mL. |
| Culture Medium | Chemically defined, serum-free medium. |
| Culture Conditions | Atmosphere: Air, 92-95%; CO2, 5-8%; Temperature: 37°C; Shaking Speed: 120-150 rpm (for 19-25 mm orbital throw). |
| Cryopreservation | 90% complete serum-free growth medium supplemented with 10% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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