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Real-time PCR or quantitative PCR (qPCR) is one of the most reliable methods of detection and quantification of specific DNA and RNA molecules. It is a more precise and accurate method of PCR because it can detect small changes in gene expression levels in a sample. It is widely used in various applications such as gene expression profiling, viral load quantification, microarray data validation, pathogen detection, and forensic analysis.
Dye-based quantitative PCR (qPCR) uses real-time fluorescence of double-stranded DNA (dsDNA)-binding dyes to measure DNA amplification that occurs during PCR. The dye exhibits weak background fluorescence that increases dramatically upon binding to dsDNA. Amplification of the target sequence thus results in an increase in fluorescence that is proportional to the amount of dsDNA present in each PCR cycle. This type of qPCR analysis requires only two sequence-specific primers, making it a fast and cost-effective method to interrogate large numbers of samples/targets.
Dye-based qPCR kits come in different formats, including kits designed for one- or two-step RT-qPCR. The One-Step RT-qPCR Kit enables reverse transcription of RNA to cDNA and qPCR amplification in a single reaction chamber. On the other hand, two-step RT-qPCR kits allow reverse transcription of RNA to cDNA in a separate reaction prior to qPCR amplification.
Creative Biogene offers a wide range of qPCR tools and kits for rapid and sensitive detection using intercalating dyes. Our kits with qPCR dye SYBR Green feature a unique combination of process controls—including built-in visual indicators—to remove variables and improve assay accuracy, specificity, and sensitivity. A novel hot-start mechanism enables you to detect a single copy of the target sequence. By delivering unparalleled accuracy, flexibility, and sensitivity, we enable researchers to quickly and accurately measure gene abundance in biological samples and track changes in gene expression in response to experimental conditions.
If you are unsure which assay and reagents are right for your application, please review our qPCR specific product pages, or contact a member of our team for a recommendation.
| Cat.No. | Product Name | Price |
|---|---|---|
| QEOC-01 | One-Step EvaGreen qRT-PCR Kit | Inquiry |
| QEQC-01 | EvaGreen qRT-PCR Kit | Inquiry |
| QSOC-01 | One-Step SYBR qRT-PCR Kit | Inquiry |
| QSOC-02 | One-Step SYBR qRT-PCR Kit (with ROX) | Inquiry |
| QSQC-01 | SYBR qRT-PCR DimerEraser Kit | Inquiry |
| QSQC-02 | SYBR qRT-PCR Kit (with ROX) | Inquiry |
| QSQC-03 | SYBR qRT-PCR Mixture Kit | Inquiry |
| QSTC-01 | Two-Step SYBR qRT-PCR Kit | Inquiry |
| QSTC-02 | Two-Step SYBR qRT-PCR Kit (with ROX) | Inquiry |
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