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Mammalian Expression System


Creative Biogene is a biotechnology company which has the expertise and experience in producing custom proteins...

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Mammalian Expression System

Creative Biogene is a biotechnology company which has the expertise and experience in producing custom proteins by mammalian expression system. Creative Biogene has sophisticated equipments, advanced technologies and highly experienced staffs which are available to assist in both transient and stable expression using a multitude of cell lines, including CHO and HEK293 expression systems. With years of development, Creative Biogene can provide customers worldwide gene expression service using mammalian expression system by establishing the best possible protocol for your project and then scale up to get the highest expression of your target protein. Creative Biogene’s goal is to provide you with the most affordable and high-quality gene expression service based on mammalian expression systems to ensure your satisfaction in a timely and professional manner.

Mammalian expression systems are the ideal choice to produce eukaryotic proteins as they are able to introduce proper protein folding, post-translational modifications, and product assembly. Although the yeast, insect and other eukaryotic cell also has the modification of glycosylation, the way of glycosylation is different from that of the human and the other mammalian cell does. Proteins expressed in mammalian cells are nearly the same with the nature proteins in the term of structure, glycosylation style and the way of glycosylation, and can be assembled into multi-subunit protein. Therefore, the products produced from mammalian systems are closest to the nature proteins in the respect of the molecular structure, character of physics and chemistry, and biological function. Mammalian expression systems are mostly used in the production of recombinant proteins, antibodies, viral vaccines virus, and viral-subunit proteins.

Applications:

  • Production of monoclonal antibody
  • Production of diagnostic and therapeutic proteins
  • Eukaryotic protein analysis, e.g. structural studies
  • Research tool proteins for crystallography

Features:

  • One-stop solution from gene synthesis/gene cloning to purified protein
  • Flexible scale options: small-scale or reproducible high-yield protein production
  • Two expression options: we offer both transient transfection and stable cell expression
  • Professional support: our experienced scientist will serve as your personal consultant from design to delivery
  • Short timeline from gene synthesis/cloning to protein recovery
  • Cost-saving: provide high quality proteins with competitive price

Creative Biogene provides gene expression service based on mammalian expression system, which includes:

  • Codon optimization for any given expression system (upon request)
  • Gene synthesis, subclone gene of interest into mammalian expression vector
  • Transient transfection into mammalian cells or construction stable cell lines
  • Stable pool selection and expression
  • Flexible scale protein expression and purification
  • Protein post processing (optional) such as purified protein tag removal, filtration sterilization, endotoxin removal, freeze-dry, N-terminal sequencing et al.)

References:

  1. Aricescu AR, et al. (2006). A time-and cost-efficient system for high-level protein production in mammalian cells. Acta Crystallographica Section D: Biological Crystallography, 62(10), 1243-1250.
  2. Geisse S. & Henke M. (2005). Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production. Journal of structural and functional genomics, 6(2), 165-170.
  3. Jayapal KP, et al. (2007). Recombinant protein therapeutics from CHO cells-20 years and counting. Chemical Engineering Progress, 103(10), 40.

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