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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | NCI-H1975 is a human non-small cell lung cancer cell line derived from a patient with lung adenocarcinoma who previously received chemotherapy. The H1975 cell line has multiple mutations in the epidermal growth factor receptor (EGFR) and TP53 genes. Specifically, H1975 cells contain the EGFR exon 21 L858R mutation. This mutation is associated with increased sensitivity to EGFR inhibitors, a class of drugs commonly used to treat NSCLC. These cells also have high levels of baseline activation of signaling pathways that promote cell growth and survival, such as the AKT and ERK pathways. Researchers use NCI-H1975 cells to study various aspects of lung cancer biology, including the molecular mechanisms of drug resistance, signaling pathways that drive tumor growth and metastasis, and the development of new therapeutic drugs. |
| Tissue | Lung |
| Disease | Adenocarcinoma; Non-small cell lung cancer |
| Morphology | Epithelial |
| Gender | Female |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | The application of NCI-H1975 cell line includes studying drug resistance mechanisms, screening potential new drugs, and investigating molecular pathways associated with EGFR mutations in lung cancer. Researchers can use this cell line to test the effectiveness of new therapies and to better understand the underlying biology of EGFR-mutated lung cancer. By utilizing the H1975 cell line, researchers can investigate the underlying molecular mechanisms that drive resistance to EGFR inhibitors, aiming to identify novel therapeutic targets and strategies to overcome this resistance. Additionally, the NCI-H1975 cell line can be used in preclinical drug development and personalized medicine studies to guide treatment decisions for patients with EGFR-mutant NSCLC. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | In addition to EGFR mutations, NCI-H1975 cells also exhibit TP53 mutations. TP53 is a key tumor suppressor gene involved in regulating cell cycle progression and DNA repair. The presence of TP53 mutations in NCI-H1975 cells is of particular interest because it is associated with more aggressive tumor behavior. Studying the functional consequences of these TP53 mutations in drug-resistant settings could reveal the interplay between TP53 status and NSCLC treatment outcome. |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Rinse the cell layer briefly with DPBS solution to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 2.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | RPMI-1640 +10% FBS +1% P/S |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃ |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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