Creative Biogene is the leading company providing Histone Deactylase (HDAC) screening and profiling services in HDAC based drug discovery field. With abundant experience for many years, we offer outstanding biochemical and cell-based HDAC assays with the most competitive price.
Creative Biogene offers the most comprehensive HDACs screening and profiling assays, including HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11, SIRT1, SIRT2, SIRT3, SIRT5, SIRT6, SIRT7, and so on. Creative Biogene provides biochemical services with the radioactivity, extraction, and chromatography aspects of other assays. Two steps on a microtiter plate are needed to analyze HDAC activity. First, purified HDAC enzyme is incubated with HDAC fluorometric substrate. Second, the deacetylation catalyzed by HDAC sensitizes the product to subsequent treatment with the developer and releases a fluorophore which is easily measured on a fluorescence reader. In addition, Creative Biogene also offers cell-based assays in a variety of tumor cell lines, such as A549 and HeLa or any cell line of your interest.
Deacetylases are enzymes that remove acetyl groups from protein substrates. HDACs are the most well studied deacetylases, which have the ability of opposing the effects of histone acetyltransferases (HATs) and restoring the positive charge of the histone tail lysine residue. Removal of the acetyl group leads to a more tightly compacted, heterochromatin conformation, thereby negatively regulating gene expression.
So far, it has been already identified at least 4 classes of HDACs. Class I HDACs include 1, 2, 3, and 8. Class II HDACs contain 4, 5, 6, 7, 9, and 10. Class III enzymes, known as sirtuins, include SIRTs 1-7. The Class IV enzyme contains only HDAC11, which has features of both Class I and II. HDACs are tightly participated in cell cycle regulation, cell proliferation, and in the development of human diseases including cancers, neurological diseases, cardiovascular and pulmonary diseases. By detection of the activity and inhibition of HDACs, it is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing, and also benefiting cancer diagnostics and therapeutics.
Fig.1 Different ways by which HDACs are recruited to gene promoters (Santiago, et al. 2007)
Creative Biogene offers a comprehensive suite of HDACs and Sirtuins screening and profiling assays. Advantages of HDACs and Sirtuins services in Creative Biogene:
- Screening for inhibitors/targets with our wide range panels of HDACs and Sirtuins
- Available for profiling and low cost high-throughput screening
- IC50 determination and single point concentration service
- High sensitivity and reproducibility
- Fast turnaround time
Our state-of-the-art labs with the best trained and most experienced staffs will work closely with you, accelerating your HDACs based drug discovery project with the highest efficiency. Creative Biogene offers unbeatable services in this field with the most competitive price. Creative Biogene is confident to be your reliable partner and dedicating to identifying your inhibitors or activators against HDACs targets with the great chance to success.
If you have any special requirements in HDACs or Sirtuins assay services, please feel free to contact us at email@example.com or 1-631-626-9181. We are looking forward to working together with your attractive projects.
- Santiago, R. and Manel, E. (2007) ‘The role of histone deacetylases (HDACs) in human cancer’, Mol Oncol., 1(1), 19-25