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Humanized murine tumor cell lines are genome-modified mouse tumor cells in which a target murine gene is replaced with its human counterpart. The humanized mouse cells can be implanted into immunocompetent mice with the same strain/genetic background, and can be applied to in vitro mechanism research and in vivo syngeneic tumor growth analysis.
Based on gene editing technology, part of the mouse F3 gene, for example, was replaced by the open reading frame of human F3 gene with a foreign promoter. At the same time, a frameshift was introduced to the coding region of the mouse F3 gene, resulting in a loss of expression of the mouse F3 protein, and a stable expression of the human F3 protein.
Figure 1. Diagram of the construction strategy of an F3 humanized cell line
Creative Biogene offers a series of humanized murine tumor cell lines that have been genetically engineered to express human immune checkpoint proteins, which can be used to evaluate the efficacy of human immune checkpoint antibodies or inhibitors in vivo and provide a cell line model for the screening of preclinical tumor immune candidates.
Cancer research has made significant advances over the years, leading to the development of novel treatment strategies and improved patient outcomes. An important aspect of cancer research involves establishing accurate and representative preclinical models to study tumor biology, drug response, and potential therapeutic targets. Humanized murine tumor cell lines have become valuable tools in cancer research due to their ability to recapitulate certain aspects of human cancer in mouse models.
Applications for humanized murine tumor cell line include:
Case Study 1
Establishment of a mouse subcutaneous tumor model using a humanized CD200 murine tumor cell line
Figure 2. Baseline characteristics of MC38 and LLC1 cancer models. (A) In vitro cultured cells were stimulated with varying concentrations of IFN-γ or control for 24 h (left panel). CD200 levels were measured in cells cultured in vitro (middle panel). After 14 days of in vivo growth, tumors were harvested and digested (right). (B) After 14 days of in vivo growth, tumors were harvested and digested. (C) Mice were injected subcutaneously with MC38 and LLC1 cells, and tumor volumes were measured every other day using calipers. Treatment with MOPC21 or mNivo was initiated once the tumors reached a certain mean volume (40 mm3 for MC38 and 20 mm3 for LLC1). (Fellermeyer M, et al., 2023).
Q: What are humanized murine tumor cell lines?
A: Humanized murine tumor cell lines are laboratory-created cell lines that are derived from murine (mouse) tumor cells in which a target murine gene is replaced with its human counterpart. This genetic modification enables the murine tumor cells to mimic certain aspects of human tumor cells, making them useful tools for studying human cancer in a laboratory setting.
Q: What are the advantages of humanized murine tumor cell lines?
A: By introducing human genes or traits, humanized murine tumor cell lines can better recapitulate the biology and behavior of human tumors compared to regular murine tumor cell lines. This allows researchers to investigate various aspects of cancer biology, such as tumor growth, metastasis, drug response, and immune interactions, in a more clinically-relevant context.
Q: What are the applications of humanized murine tumor cell lines in drug development?
A: Humanized murine tumor cell lines are commonly used in preclinical cancer research to evaluate novel anticancer therapies, study tumor-host interactions, and develop new treatment strategies. They can also be instrumental in the development of personalized medicine approaches by providing a more realistic experimental model for testing drug efficacy and toxicity in a human tumor microenvironment.
Q: Can you provide custom humanized mouse tumor cell lines?
A: Yes. Our gene editing team can generate humanized murine cancer cell lines for your specific needs.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RH0001M | Humanized CD274/CD47 Murine Tumor Cell Line | Inquiry |
| CSC-RH0002M | Humanized CD274/PCSK9 Murine Tumor Cell Line | Inquiry |
| CSC-RH0003M | Humanized LRRC15 Murine Tumor Cell Line | Inquiry |
| CSC-RH0004M | Humanized EFEMP1 Murine Tumor Cell Line | Inquiry |
| CSC-RH0005M | Humanized MMP8 Murine Tumor Cell Line | Inquiry |
| CSC-RH0006M | Humanized MSLN Murine Tumor Cell Line | Inquiry |
| CSC-RH0007M | Humanized CDH17 Murine Tumor Cell Line | Inquiry |
| CSC-RH0008M | Humanized CD200 Murine Tumor Cell Line | Inquiry |
| CSC-RH0009M | Humanized ERBB2 Murine Tumor Cell Line | Inquiry |
| CSC-RH0010M | Humanized CD274/ROR1 Murine Tumor Cell Line | Inquiry |
| CSC-RH0011M | Humanized ADAM9 Murine Tumor Cell Line | Inquiry |
| CSC-RH0012M | Humanized ITGA7 Murine Tumor Cell Line | Inquiry |
| CSC-RH0013M | Humanized CA12 Murine Tumor Cell Line | Inquiry |
| CSC-RH0014M | Humanized VCAM1 Murine Tumor Cell Line | Inquiry |
| CSC-RH0015M | Humanized MPL Murine Tumor Cell Line | Inquiry |
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