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Herpes Simplex Virus (HSV) Service

Creative Biogene is a world leader in the area of virus engineering and packaging. With the deep knowledge on the genetics and molecular biology of HSV, along with our state-of-the-art facilities, scientists at Creative Biogene have developed an excellent HSV production platform.

Herpes simplex virus (HSV) is an enveloped, large linear and double-stranded DNA (~150 kb) virus in the family Herpesviridae. There are two HSV species: Herpes Simplex Virus Type I (HSV-1) and Herpes Simplex Virus Type II (HSV-2), both of which can infect humans and cause diseases. HSV genome replicates by means of a rolling circle mechanism which forms head-to-tail concatamers. In the large genome of HSV, about half of the sequences are non-essential genes, which means they can be replaced with one or multiple transgenes.

Figure 1. Herpes simplex virus type 1 genome

HSV-1 has been well-characterized and engineered as a gene delivery vector both in research and therapy. Three classes of recombinant vectors have been developed based on HSV-1, which include replication-competent viruses, replication-defective viruses and amplicons. The HSV system we have established is based on HSV-1 H129 strain which is originally isolated from a patient. Through genetic engineering and optimization, our HSV system are able to produce high titer viruses allowing high level transgene expression in infected host cells.

Advantages

  • Deliver transgenes into a wide range of cells, including neurons with higher efficiency compared with other systems
  • Long-term and high-level transgene expression in host cells
  • Large DNA capacity

Applications

  • Gene transfer and function research
  • Act as a powerful tool in transynaptic neural tracing studies
  • Develop oncolytic vectors that could be used in cancer treatment

Figure 2. Fluorescent image of cells infected with rHSV1-EGFP

Our Service Includes

  • Synthesize and subclone your DNA of interest into our HSV plasmid
  • Co-transfect of the HSV plasmid and HSV genome into packaging cells
  • Harvest recombinant viruses
  • Perform virus titration (PFU)

References

  1. Kuypers HG, Ugolini G. Viruses as transneuronal tracers. Trends Neurosci. 1990;2:71–5.
  2. Lo L, Anderson DJ. A Cre-dependent, anterograde transsynaptic viral tracer for mapping output pathways of genetically marked neurons. Neuron. 2011 Dec 22;72(6):938-50.
  3. McGovern AE, Davis-Poynter N, Rakoczy J, Phipps S, Simmons DG, Mazzone SB. Anterograde neuronal circuit tracing using a genetically modified herpes simplex virus expressing EGFP. J Neurosci Methods. 2012 Jul 30;209(1):158-67.

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