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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HCC70 is an epithelial cell line isolated from primary ductal carcinoma of a 49-year-old black woman in 1992, and it took 44 months for the cells to establish. The HCC70 cell line is derived from human triple-negative breast cancer (TNBC), one of the most aggressive subtypes of breast cancer that lacks expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). This cell line encompasses many of the molecular features and genetic abnormalities inherent to TNBC, underscoring its value for preclinical studies of this challenging subtype of breast cancer. In vitro and in vivo experiments with HCC70 provide insights into TNBC's aggressive growth patterns, metastatic propensity, stromal interactions, and response to various therapeutic agents. Researchers rely on the HCC70 model to dissect the molecular basis of TNBC and develop and evaluate treatment strategies, particularly those that target the unique vulnerabilities of triple-negative tumors. |
| Tissue | Breast; Duct; Mammary gland |
| Disease | TNM stage IIIA, grade 3, primary ductal carcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 49 years |
| Product Format | Frozen |
| Growth Mode | Adherent, attached medium-sized epithelial cells without floating cells |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer biology research 2. Drug screening and development 3. Genetic studies 4. Signal transduction pathways research 5. Research on drug resistance mechanisms 6. Immunology research 7. Gene editing and functional studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | HCC70 cells are poorly differentiated. The tumor was classified as TNM stage IIIA grade 3 invasive ductal carcinoma with metastasis in 4 of 17 lymph nodes. These cells overexpress p53 and are negative for Her2-neu oncogene expression. HCC70 was positive for the epithelial cell-specific markers epithelial glycoprotein 2 (EGP2) and cytokeratin 19. |
| Characteristics | |
| Genes Expressed | Epithelial glycoprotein 2 (EGP2); cytokeratin 19 |
| Expression Markers | Progesterone receptor, not expressed |
| Karyotype | Polyploid |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. 1. Rinse the cell layer briefly with calcium- and magnesium-free PBS to remove all traces of serum containing trypsin inhibitors. 2. Add 1.0~3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5~15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 4. Transfer the cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. 5. Discard the supernatant and resuspend the cells in fresh growth medium. 6. Add appropriate aliquots of cell suspension to new culture vessels. Maintain cultures at a cell concentration between 4x104 and 4 x 105 cells/cm2. 7. Place the culture vessel into a 37°C incubator. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose with fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0159 | Cas9 Stable Cell Line - HCC70 | Inquiry |
| CSC-RR0213 | Luc/GFP Reporter Cell Line-HCC70 | Inquiry |
| CSC-RR0219 | GFP Reporter Cell Line-HCC70 | Inquiry |
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